Cash, Darian D. Cohen-Zontag, Osnat Kim, Nak-Kyoon Shefer, Kinneret Brown, Yogev Ulyanov, Nikolai B. Tzfati, Yehuda Feigon, Juli 2024-01-20T12:02:07Z 2024-01-20T12:02:07Z 2021-08-31 2013-07-02 0027-8424 https://pubs.kist.re.kr/handle/201004/127877 Telomerase is a ribonucleoprotein complex that extends the 3' ends of linear chromosomes. The specialized telomerase reverse transcriptase requires a multidomain RNA (telomerase RNA, TER), which includes an integral RNA template and functionally important template-adjacent pseudoknot. The structure of the human TER pseudoknot revealed that the loops interact with the stems to form a triple helix shown to be important for activity in vitro. A similar triple helix has been predicted to form in diverse fungi TER pseudoknots. The solution NMR structure of the Kluyveromyces lactis pseudoknot, presented here, reveals that it contains a long pyrimidine motif triple helix with unexpected features that include three individual bulge nucleotides and a C+circle G-C triple adjacent to a stem 2-loop 2 junction. Despite significant differences in sequence and base triples, the 3D shape of the human and K. lactis TER pseudoknots are remarkably similar. Analysis of the effects of nucleotide substitutions on cell growth and telomere lengths provides evidence that this conserved structure forms in endogenously assembled telomerase and is essential for telomerase function in vivo. English NATL ACAD SCIENCES DYSKERATOSIS-CONGENITA TEMPLATE BOUNDARY MAINTENANCE DYNAMICS ELEMENT CONSERVATION BIOGENESIS REVEALS BINDING CANCER Pyrimidine motif triple helix in the Kluyveromyces lactis telomerase RNA pseudoknot is essential for function in vivo Article 10.1073/pnas.1309590110 1 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.110, no.27, pp.10970 - 10975 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 110 27 10970 10975 scie scopus 000321978000034 Multidisciplinary Sciences Science & Technology - Other Topics Article DYSKERATOSIS-CONGENITA TEMPLATE BOUNDARY MAINTENANCE DYNAMICS ELEMENT CONSERVATION BIOGENESIS REVEALS BINDING CANCER RNA triplex yeast RNA structure Hoogsteen