Yoon, Hong Yeol Koo, Heebeom Choi, Ki Young Kwon, Ick Chan Choi, Kuiwon Park, Jae Hyung Kim, Kwangmeyung 2024-01-20T12:02:45Z 2024-01-20T12:02:45Z 2021-09-05 2013-07 0142-9612 https://pubs.kist.re.kr/handle/201004/127910 One of the major hurdles of the nanoparticles as drug carriers is the unintended burst release of loaded drugs during blood circulation. To surmount this issue, we developed photo-crosslinked hyaluronic acid nanoparticles (c-HANPs) with improved stability for tumor-targeted drug delivery. They were readily prepared via UV-triggered chemical crosslinking with the acrylate groups in the polymer backbone. The size of c-HANPs was not much different from that of uncrosslinked HANPs. However, c-HANPs exhibited significantly high stability in a physiological buffer and released the loaded drug, paclitaxel (PTX), in a sustained manner. It is noteworthy that the drug release rate from c-HANPs remarkably increased in the presence of hyaluronidase, an enzyme abundant at the intracellular compartments of the tumor cells. It was found from in vitro cellular uptake tests that c-HANPs were rapidly taken up by the tumor cells via the receptor (CD44)-mediated endocytosis, which was not inhibited by photo-crosslinking. In non-invasive animal imaging results, they showed higher tumor-targeting ability than uncrosslinked HANPs because high stability of c-HANPs enabled their long circulation in the body. Owing to the sustained release of the drug and enhanced tumor-targeting ability, c-HANPs showed higher therapeutic efficacy compared to free PTX and uncrosslinked HANPs. These data implied the promising potential of c-HANP as tumor-targeting drug carriers and demonstrated the remarkable effect of the improved stability upon the biodistribution and therapeutic efficacy of drug-loaded nanoparticles. (C) 2013 Elsevier Ltd. All rights reserved. English ELSEVIER SCI LTD POLYMERIC MICELLES CANCER-THERAPY THERAPEUTICS NANOPROBES DIAGNOSIS SYSTEMS Photo-crosslinked hyaluronic acid nanoparticles with improved stability for in vivo tumor-targeted drug delivery Article 10.1016/j.biomaterials.2013.03.050 1 BIOMATERIALS, v.34, no.21, pp.5273 - 5280 BIOMATERIALS 34 21 5273 5280 scie scopus 000319630000037 2-s2.0-84877064712 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article POLYMERIC MICELLES CANCER-THERAPY THERAPEUTICS NANOPROBES DIAGNOSIS SYSTEMS Polymeric nanoparticle Drug delivery Hyaluronic acid Crosslinking Stability