Song, Mi-Kyung Song, Mee Choi, Han-Seam Park, Yong-Keun Ryu, Jae Chun 2024-01-20T12:03:37Z 2024-01-20T12:03:37Z 2021-09-05 2013-06-20 1976-0280 https://pubs.kist.re.kr/handle/201004/127951 The aim of this study was to determine the transcriptomic response in workers exposed to volatile organic compounds (VOCs). As blood is one of the most easily accessible sources of RNA for analyzing environmental exposure, we identified and validated characteristic gene expression profiles of human whole blood in patients exposed to VOCs to determine if the transcriptomic responses of circulating blood cells reflected exposure to environmental toxicants. The findings demonstrated that the characteristic molecular signatures can help discriminate and predict the toxicant type. To evaluate the changes in gene expression levels in workers exposed to VOCs (toluene [TOL] and trichloroethylene [TCE]), We analyzed the genome-wide expression profiles of whole bloods of TOL & TCE exposed workers and unexposed healthy subjects. Unsupervised gene expression analysis resulted in a characteristic molecular signature for each toxicant, and a supervised analysis identified 9,724 outlier genes as distinct molecular signatures distinguishing VOC-exposed workers from healthy controls. Further analysis of the multi-classification revealed 378 genes as surrogate markers for predicting each VOC with 100% accuracy. Our results suggest that these expression signatures can be used as predictable and discernible surrogate markers for screening VOC exposure. Furthermore, the use of these markers can be more widely applied in combination with more traditional techniques for assessing and screening toxic exposure to environmental VOCs. English KOREAN BIOCHIP SOCIETY-KBCS POLYCYCLIC AROMATIC-HYDROCARBONS LONG-TERM TOXICOGENOMICS CANCER CARCINOGENICITY PREDICTION BIOMARKERS TOXICOLOGY TOXICANTS TOXICITY Discovery of a characteristic molecular signature by microarray analysis of whole-blood gene expression in workers exposed to volatile organic compounds Article 10.1007/s13206-013-7205-9 1 BIOCHIP JOURNAL, v.7, no.2, pp.112 - 135 BIOCHIP JOURNAL 7 2 112 135 scie scopus kci other ART001775742 000320909400005 2-s2.0-84879700725 Biochemical Research Methods Chemistry, Analytical Nanoscience & Nanotechnology Biochemistry & Molecular Biology Chemistry Science & Technology - Other Topics Article POLYCYCLIC AROMATIC-HYDROCARBONS LONG-TERM TOXICOGENOMICS CANCER CARCINOGENICITY PREDICTION BIOMARKERS TOXICOLOGY TOXICANTS TOXICITY Molecular signature Volatile organic compounds Toluene Trichloroethylene Transcriptomics