Han, Hwa Seung Lee, Jungmin Kim, Hyun Ryoung Chae, Su Young Kim, Minwoo Saravanakumar, Gurusamy Yoon, Hong Yeol You, Dong Gil Ko, Hyewon Kim, Kwangmeyung Kwon, Ick Chan Park, Jae Chan Park, Jae Hyung 2024-01-20T12:03:58Z 2024-01-20T12:03:58Z 2021-09-05 2013-06-10 0168-3659 https://pubs.kist.re.kr/handle/201004/127969 The in vivo stability and tumor targetability of self-assembled polymeric nanoparticles are crucial for effective drug delivery. In this study, to develop biostable nanoparticles with high tumor targetability, poly(ethylene glycol)-conjugated hyaluronic acid nanoparticles (PEG-HANPs) were mineralized through controlled deposition of inorganic calcium and phosphate ions on the nanoparticular shell via a sequential addition method. The resulting nanoparticles (M-PEG-HANPs) had a smaller size (153.7 +/- 4.5 nm) than bare PEG-HANPs (265.1 +/- 9.5 nm), implying that mineralization allows the formation of compact nanoparticles. Interestingly, when the mineralized nanoparticles were exposed to acidic buffer conditions (<pH 6.5), their sizes increased rapidly due to dissolution of the inorganic minerals. Doxorubicin (DOX), chosen as the model anticancer drug, was effectively encapsulated into the bare and mineralized nanoparticles. For bare PEG-HANPs, DOX was released in a sustained manner and its release rate was not dependent on the pH of the solution. On the other hand, DOX release from M-PEG-HANPs was pH-dependent: i.e. DOX was slowly released from nanoparticles under physiological condition (pH 7.4), whereas its release rates were much higher at mildly acidic environments (<pH 6.5). From in vivo biodistribution study, it was found that M-PEG-HANPs could reach the tumor site more effectively than bare PEG-HANPs. The antitumor efficacy of DOX-loaded nanoparticles was evaluated after systemic administration into the tumor-bearing mice. Of the samples tested, the most effective antitumor efficacy was observed for DOX-loaded M-PEG-HANPs. Overall, these results suggest that M-PEG-HANPs could be a promising carrier for an anticancer drug. (C) 2013 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV BLOCK-COPOLYMER MICELLES CANCER-THERAPY THERAPEUTICS NANOCARRIERS STABILITY RECEPTOR Robust PEGylated hyaluronic acid nanoparticles as the carrier of doxorubicin: Mineralization and its effect on tumor targetability in vivo Article 10.1016/j.jconrel.2013.02.022 1 JOURNAL OF CONTROLLED RELEASE, v.168, no.2, pp.105 - 114 JOURNAL OF CONTROLLED RELEASE 168 2 105 114 scie scopus 000319499200001 2-s2.0-84876190170 Chemistry, Multidisciplinary Pharmacology & Pharmacy Chemistry Pharmacology & Pharmacy Article BLOCK-COPOLYMER MICELLES CANCER-THERAPY THERAPEUTICS NANOCARRIERS STABILITY RECEPTOR Mineralization Hyaluronic acid Nanoparticles Doxorubicin Stability