Lee, Aeju Choi, Sung-Jae Park, Kyeongsoon Park, Jong Woong Kim, Kwangmeyung Choi, Kuiwon Yoon, Soo-Young Youn, Inchan 2024-01-20T12:04:55Z 2024-01-20T12:04:55Z 2021-09-04 2013-06 1043-1802 https://pubs.kist.re.kr/handle/201004/128017 The activity of rheumatoid arthritis (RA) correlates with the expression of proteases. Among several proteases, matrix metalloproteinase-3 (MMP-3) is one of the biological markers used to diagnose RA. The active form of MMP-3 is a key enzyme involved in RA-associated destruction of cartilage and bone. Thus, detection of active MMP-3 in serum or in vivo is very important for early diagnosis of RA. In this study, a soluble MMP-3 probe was prepared to monitor RA progression by detecting expression of active MMP-3 in collagen-induced arthritis (CIA) mice in vivo in both serum and fibroblast-like synoviocytes (FLSs). The MMP-3 probe exhibited strong sensitivity to MMP-3 and moderate sensitivity to MMP-7 at nanomolecular concentrations, but was not sensitive to other MMPs such as MMP-2, MMP-9, and MMP-13. In an optical imaging study, the MMP-3 probe produced early and strong NIR fluorescence signals prior to observation of erythema and swelling in CIA mice. The MMP-3 probe was able to rapidly and selectively detect and monitor active MMP-3 in diluted serum from CIA mice. Furthermore, histological data demonstrated that activated FLSs in arthritic knee joints expressed active MMP-3. Together, our results demonstrated that the MMP-3 probe may be useful for detecting active MMP-3 for diagnosis of RA. More importantly, the MMP-3 probe was able to detect active MMP-3 in diluted serum with high sensitivity. Therefore, the MMP-3 probe developed in this study may be a very promising probe, useful as a biomarker for early detection and diagnosis of RA English AMER CHEMICAL SOC EARLY RHEUMATOID-ARTHRITIS IMMOBILIZED DIAGNOSTIC KIT INFLAMMATORY ARTHRITIDES TISSUE INHIBITORS DISEASE-ACTIVITY SYNOVIAL-FLUID JOINT DAMAGE OSTEOARTHRITIS PROGRESSION MMP-3 Detection of Active Matrix Metalloproteinase-3 in Serum and Fibroblast-Like Synoviocytes of Collagen-Induced Arthritis Mice Article 10.1021/bc4001273 1 BIOCONJUGATE CHEMISTRY, v.24, no.6, pp.1068 - 1074 BIOCONJUGATE CHEMISTRY 24 6 1068 1074 scie scopus 000320898900026 2-s2.0-84879349241 Biochemical Research Methods Biochemistry & Molecular Biology Chemistry, Multidisciplinary Chemistry, Organic Biochemistry & Molecular Biology Chemistry Article EARLY RHEUMATOID-ARTHRITIS IMMOBILIZED DIAGNOSTIC KIT INFLAMMATORY ARTHRITIDES TISSUE INHIBITORS DISEASE-ACTIVITY SYNOVIAL-FLUID JOINT DAMAGE OSTEOARTHRITIS PROGRESSION MMP-3 Rheumatoid arthritis active MMP-3 Fibroblast-like synoviocyte serum Collagen-induced arthritis