Song, Mee Song, Mi-Kyung Choi, Han-Seam Ryu, Jae-Chun 2024-01-20T12:04:57Z 2024-01-20T12:04:57Z 2021-09-04 2013-06 0340-5761 https://pubs.kist.re.kr/handle/201004/128018 Iodothyronine deiodinase types I, II, and III (D1, D2, and D3, respectively), which constitute a family of selenoenzymes, activate and inactivate thyroid hormones through the removal of specific iodine moieties from thyroxine and its derivatives. These enzymes are important in the biological effects mediated by thyroid hormones. The expression of activating and inactivating deiodinases plays a critical role in a number of cell systems, including the neuronal system, during development as well as in adult vertebrates. To investigate deiodinase-disrupting chemicals based on transcriptomic responses, we examined differences in gene expression profiles between T3-treated and deiodinase-knockdown SH-SY5Y cells using microarray analysis and quantitative real-time RT-PCR. A total of 1,558 genes, consisting of 755 upregulated and 803 downregulated genes, were differentially expressed between the T3-treated and deiodinase-knockdown cells. The expression levels of 10 of these genes (ID2, ID3, CCL2, TBX3, TGOLN2, C1orf71, ZNF676, GULP1, KLF9, and ITGB5) were altered by deiodinase-disrupting chemicals (2,3,7,8-tetrachlorodibenzo-p-dioxin, polychlorinated biphenyls, propylthiouracil, iodoacetic acid, methylmercury, beta-estradiol, methimazole, 3-methylcholanthrene, aminotriazole, amiodarone, cadmium chloride, dimethoate, fenvalerate, octylmethoxycinnamate, iopanoic acid, methoxychlor, and 4-methylbenzylidene-camphor). These genes are potential biomarkers for detecting deiodinase deficiency and predicting their effects on thyroid hormone production. English SPRINGER HEIDELBERG THYROID-HORMONE METABOLISM IODOTHYRONINE 5-MONODEIODINASE ACTIVITY GENE-EXPRESSION IN-VITRO IDENTIFICATION INHIBITION EXPOSURE CADMIUM BRAIN LIVER Monitoring of deiodinase deficiency based on transcriptomic responses in SH-SY5Y cells Article 10.1007/s00204-013-1018-4 1 ARCHIVES OF TOXICOLOGY, v.87, no.6, pp.1103 - 1113 ARCHIVES OF TOXICOLOGY 87 6 1103 1113 scie scopus 000319413900017 2-s2.0-84878679379 Toxicology Toxicology Article THYROID-HORMONE METABOLISM IODOTHYRONINE 5-MONODEIODINASE ACTIVITY GENE-EXPRESSION IN-VITRO IDENTIFICATION INHIBITION EXPOSURE CADMIUM BRAIN LIVER Deiodinase Disruption Biomarker Chemical Microarray