Gu, M.-Y. Kwon, H.C. Song, M.O. Ko, H. Cha, J.-W. Lee, W.J. Yang, H.O. 2024-01-20T12:05:06Z 2024-01-20T12:05:06Z 2021-09-02 2013-06 1226-3907 https://pubs.kist.re.kr/handle/201004/128027 The autophagy-lysosomal pathway is an important protein degradation system, and its dysfunction has been implicated in a number of neurodegenerative diseases, including Parkinson's disease. Raphani Semen, one of the herbs of Yeoldahanso-tang (YH), has neuroprotective effects via the autophagy pathway. The activity-guided method was used to isolate and identify the components of Raphani Semen. In this experiment, the total extract of Raphani Semen was partitioned to n-butanol, methylene chloride, and water fractions. Flow cytometry data showed that only the water fraction showed autophagy-inducing activity in vitro. Compounds 1 and 2 were isolated from this water fraction by preparative HPLC separation. The structures of compounds 1 and 2 were identified as stachyose and raffinose, respectively, by the analysis of various spectral data (1H NMR, 13C NMR, and MS) and comparisons with standard stachyose and raffinose. Of these two compounds, raffinose showed autophagy-inducing activity in PC12 cells through the mTOR pathway. English butanol dichloromethane mammalian target of rapamycin oligosaccharide plant extract raffinose rapamycin Raphani semen extract stachyose unclassified drug water article autophagy Brassicaceae cell viability concentration (parameters) controlled study drug isolation flow cytometry high performance liquid chromatography in vitro study nonhuman PC12 cell Raphani semen signal transduction Isolation and identification of an autophagy-inducing compound from Raphani Semen Article 1 Natural Product Sciences, v.19, no.3, pp.242 - 250 Natural Product Sciences 19 3 242 250 scopus kci ART001804682 2-s2.0-84887691349 Article butanol dichloromethane mammalian target of rapamycin oligosaccharide plant extract raffinose rapamycin Raphani semen extract stachyose unclassified drug water article autophagy Brassicaceae cell viability concentration (parameters) controlled study drug isolation flow cytometry high performance liquid chromatography in vitro study nonhuman PC12 cell Raphani semen signal transduction Autophagy Cruciferae Raffinose Raphani semen Raphanus sativa L.