Kim, Yeon Seok Song, Min Young Jurng, Jongsoo Kim, Byoung Chan 2024-01-20T12:30:59Z 2024-01-20T12:30:59Z 2021-09-05 2013-05-01 0003-2697 https://pubs.kist.re.kr/handle/201004/128075 Aptamers are powerful capturing probes against various targets such as proteins, small organic compounds, metal ions, and even cells. In this study, we isolated and characterized single-stranded DNA (ssDNA) aptamers against Escherichia coli. A total of 28 ssDNAs were isolated after 10 rounds of selection using a bacterial cell-SELEX (systematic evolution of ligands by exponential enrichment) process. Other bacterial species (Klebsiella pneumoniae, Citrobacter freundii, Enterobacter aerogenes, and Staphylococcus epidermidis) were used for counter selection to enhance the selectivity of ssDNA aptamers against E. coli. Finally, four ssDNA aptamers showed high affinity and selectivity to E. coli, The dissociation constants (K-d) of these four ssDNA aptamers to E. coli were estimated to range from 12.4 to 25.2 nM. These aptamers did not bind to other bacterial species, including four counter cells, but they showed affinity to different E. coli strains. The binding of these four aptamers to E. coli was observed directly by fluorescence microscopy. (C) 2013 Elsevier Inc. All rights reserved. English ACADEMIC PRESS INC ELSEVIER SCIENCE SWITCHING SIGNALING APTAMERS IN-VITRO SELECTION SEQUENCE BINDING MOLECULES STRATEGIES PATHOGENS SAMPLES Isolation and characterization of DNA aptamers against Escherichia coli using a bacterial cell-systematic evolution of ligands by exponential enrichment approach Article 10.1016/j.ab.2013.01.014 1 ANALYTICAL BIOCHEMISTRY, v.436, no.1, pp.22 - 28 ANALYTICAL BIOCHEMISTRY 436 1 22 28 scie scopus 000316583800005 2-s2.0-84874398911 Biochemical Research Methods Biochemistry & Molecular Biology Chemistry, Analytical Biochemistry & Molecular Biology Chemistry Article SWITCHING SIGNALING APTAMERS IN-VITRO SELECTION SEQUENCE BINDING MOLECULES STRATEGIES PATHOGENS SAMPLES Bacteria cell-SELEX DNA aptamers E. coli Fluorescence