Yoon, Hyung Ho Min, Joongkee Shin, Nani Kim, Yong Hwan Kim, Jin-Mo Hwang, Yu-Shik Suh, Jun-Kyo Francis Hwang, Onyou Jeon, Sang Ryong 2024-01-20T12:31:08Z 2024-01-20T12:31:08Z 2022-01-25 2013-05 1673-5374 https://pubs.kist.re.kr/handle/201004/128082 Transplantation of neural stem cells has been reported as a possible approach for replacing impaired dopaminergic neurons. In this study, we tested the efficacy of early-stage human dental papilla-derived stem cells and human brain-derived neural stem cells in rat models of 6-hydroxydopamine-induced Parkinson's disease. Rats received a unilateral injection of 6-hydroxydopamine into right medial forebrain bundle, followed 3 weeks later by injections of PBS, early-stage human dental papilla-derived stem cells, or human brain-derived neural stem cells into the ipsilateral striatum. All of the rats in the human dental papilla-derived stem cell group died from tumor formation at around 2 weeks following cell transplantation. Postmortem examinations revealed homogeneous malignant tumors in the striatum of the human dental papilla-derived stem cell group. Stepping tests revealed that human brain-derived neural stem cell transplantation did not improve motor dysfunction. In apomorphine-induced rotation tests, neither the human brain-derived neural stem cell group nor the control groups (PBS injection) demonstrated significant changes. Glucose metabolism in the lesioned side of striatum was reduced by human brain-derived neural stem cell transplantation. [F-18]-FP-CIT PET scans in the striatum did not demonstrate a significant increase in the human brain-derived neural stem cell group. Tyrosine hydroxylase (dopaminergic neuronal marker) staining and G protein-activated inward rectifier potassium channel 2 (A9 dopaminergic neuronal marker) were positive in the lesioned side of striatum in the human. brain-derived neural stem cell group. The use of early-stage human dental papilla-derived stem cells confirmed its tendency to form tumors. Human brain-derived neural stem cells could be partially differentiated into dopaminergic neurons, but they did not secrete dopamine. English SHENYANG EDITORIAL DEPT NEURAL REGENERATION RES Are human dental papilla-derived stem cell and human brain-derived neural stem cell transplantations suitable for treatment of Parkinson's disease? Article 10.3969/j.issn.1673-5374.2013.13.004 1 NEURAL REGENERATION RESEARCH, v.8, no.13, pp.1190 - 1200 NEURAL REGENERATION RESEARCH 8 13 1190 1200 N scie scopus 000319852800004 2-s2.0-84880073682 Cell Biology Neurosciences Cell Biology Neurosciences & Neurology Article MULTIPLE SYSTEM ATROPHY RAT MODEL SUBTHALAMIC NUCLEUS IN-VITRO DIFFERENTIATION MICE 6-HYDROXYDOPAMINE STIMULATION DOPAMINE LEVODOPA neural regeneration stem cells cell transplantation glucose metabolism human brain-derived neural stem cells human dental papilla-derived stem cells Parkinson&apos s disease positron emission tomography grants-supported paper neuroregeneration