Pribylka, Adam Almeida, Ana Vanessa Altmeyer, Matthias O. Petr, Jan Sevcik, Juraj Manz, Andreas Neuzil, Pavel 2024-01-20T12:31:13Z 2024-01-20T12:31:13Z 2022-01-10 2013-05 1473-0197 https://pubs.kist.re.kr/handle/201004/128086 Here we present the results of Bacillus subtilis spores breaking using superheating. The spore sample was pumped through the open-ended capillary tube mounted across the heated zone. We investigated the influence of temperature in the range 120-180 degrees C. The heat exposure was controlled by the length of the heated zone, the inner diameter of the capillary and the sample flow rate. We found that spore treatment above 120 degrees C resulted in the release of DNA within 20 s. English Royal Society of Chemistry SAMPLE PREPARATION METHOD BACTERIAL-SPORES BACILLUS-SUBTILIS ANTHRAX SPORES DNA ANALYSIS RESISTANCE CELLS HEAT GERMINATION DISRUPTION Fast spore breaking by superheating Article 10.1039/c3lc41305e 1 Lab on a Chip, v.13, no.9, pp.1695 - 1698 Lab on a Chip 13 9 1695 1698 scie scopus 000316962700005 Biochemical Research Methods Chemistry, Multidisciplinary Chemistry, Analytical Nanoscience & Nanotechnology Instruments & Instrumentation Biochemistry & Molecular Biology Chemistry Science & Technology - Other Topics Instruments & Instrumentation Article SAMPLE PREPARATION METHOD BACTERIAL-SPORES BACILLUS-SUBTILIS ANTHRAX SPORES DNA ANALYSIS RESISTANCE CELLS HEAT GERMINATION DISRUPTION