Park, Cho Rong You, Dong-Joo Kim, Dong-Kyu Moon, Mi Jin Lee, Cheolju Oh, Seung-Hyun Ahn, Curie Seong, Jae Young Hwang, Jong-Ik 2024-01-20T12:31:30Z 2024-01-20T12:31:30Z 2021-09-05 2013-05 0730-2312 https://pubs.kist.re.kr/handle/201004/128102 CXCL14 is a chemokine family member that is involved in various cellular responses in addition to immune cell activation. Although constitutive CXCL14 expression in normal epithelial cells may help protect against infection by activating immune systems, its expression in cancer cells has raised controversy regarding its possible role in tumorigenesis. However, the underlying mechanisms for this disparity remain unknown. Investigation of cellular CXCL14 binding properties might increase our understanding of the peptide's roles in tumorigenesis. In the present study, we found that CXCL14 binds to various cell types. Interestingly, binding to NCI-H460 cells was prevented by heparan sulfate and N-acetyl neuraminic acid. Next, we examined effect of CXCL14 binding in NCI-H460 and NCI-H23. CXCL14 enhanced proliferation and migration in NCI-H460 but had no effect on NCI-H23. A reporter gene assay with various transcription factor response elements revealed that only nuclear factor-B (NF-B) signaling was activated by CXCL14 in NCI-H460 cells, which was blocked by BAPTA-AM, TPCA-1, and brefeldin A. Exogenous expression of some glycoproteins such as syndecan-4, podoplanin, and CD43 in these cells enhanced CXCL14 binding and NF-B activity. Collectively, these results demonstrate that CXCL14 binding to glycoproteins harboring heparan sulfate proteoglycans and sialic acids leads proliferation and migration of some cancer cells. J. Cell. Biochem. 114: 10841096, 2013. (c) 2012 Wiley Periodicals, Inc. English WILEY-BLACKWELL DENDRITIC CELLS IN-VIVO POTENT INHIBITOR CHEMOKINE BRAK EXPRESSION TISSUE ANGIOGENESIS RESISTANCE HYALURONAN SUGGESTS CXCL14 enhances proliferation and migration of NCI-H460 human lung cancer cells overexpressing the glycoproteins containing heparan sulfate or sialic acid Article 10.1002/jcb.24449 1 JOURNAL OF CELLULAR BIOCHEMISTRY, v.114, no.5, pp.1084 - 1096 JOURNAL OF CELLULAR BIOCHEMISTRY 114 5 1084 1096 scie scopus 000316332200014 2-s2.0-84875146604 Biochemistry & Molecular Biology Cell Biology Biochemistry & Molecular Biology Cell Biology Article DENDRITIC CELLS IN-VIVO POTENT INHIBITOR CHEMOKINE BRAK EXPRESSION TISSUE ANGIOGENESIS RESISTANCE HYALURONAN SUGGESTS CXCL14 HEPARAN SULFATE PROTEOGLYCAN SIALIC ACID GLYCOPROTEIN MIGRATION NF-B