Kim, Ji Hyun Park, In Su Park, Yongdoo Jung, Youngmee Kim, Soo Hyun Kim, Sang-Heon 2024-01-20T12:31:43Z 2024-01-20T12:31:43Z 2021-09-05 2013-05 1465-3249 https://pubs.kist.re.kr/handle/201004/128113 Background aims. To successfully treat myocardial infarction (MI), blood must be resupplied to the ischemic myocardium by inducing angiogenesis. Many studies report enhanced angiogenesis using stem cells; however, the therapeutic efficacy of cell transplant remains low because transplanted cells may not survive, be retained at the site of transplant, or develop into vascular tissue. In this study, we assessed the therapeutic potential of three-dimensional cell masses (3DCM) composed of human adipose-derived stem cells (hASC) in a rat MI model. Methods. For formation of 3DCM, hASC were cultured on a substrate with immobilized fibroblast growth factor 2. The morphology and phenotypes of 3DCM were analyzed 1 day after culture. The cells (hASC and 3DCM, 5 x 10(5) cells) were injected into ischemic regions after ligation of the left coronary artery (n = 6 in each group). Cell retention ratio, therapeutic efficacy and vascularization were evaluated 4 weeks after transplant. Results. A spheroid-type 3DCM, which included vascular cells (CD34(+)/CD31(+)/KDR+/alpha-SMA(+)) with high production of human vascular endothelial growth factor, was obtained. Infarct size and cardiomyocyte apoptosis were reduced in the 3DCM-injected group compared with the hASC-injected group. The retention ratio of hASC was 14-fold higher in the 3DCM-injected group. Many transplanted cells differentiated into endothelial and smooth muscle cells and formed vascular networks incorporated into host vessels. Conclusions. Transplant of 3DCM may be useful for angiogenic cell therapy to treat MI. English ELSEVIER SCI LTD STROMAL CELLS MYOCARDIAL-INFARCTION CARDIAC-FUNCTION GROWTH PROGENITOR SPHEROIDS DIFFERENTIATION SECRETION DELIVERY TRANSPLANTATION Therapeutic angiogenesis of three-dimensionally cultured adipose-derived stem cells in rat infarcted hearts Article 10.1016/j.jcyt.2012.11.016 1 CYTOTHERAPY, v.15, no.5, pp.542 - 556 CYTOTHERAPY 15 5 542 556 scie scopus 000317370900004 2-s2.0-84875752891 Cell & Tissue Engineering Biotechnology & Applied Microbiology Cell Biology Hematology Medicine, Research & Experimental Cell Biology Biotechnology & Applied Microbiology Hematology Research & Experimental Medicine Article STROMAL CELLS MYOCARDIAL-INFARCTION CARDIAC-FUNCTION GROWTH PROGENITOR SPHEROIDS DIFFERENTIATION SECRETION DELIVERY TRANSPLANTATION angiogenesis cell adhesion substrate differentiation human adipose-derived stem cells myocardial infarction stem cell transplant three-dimensional cell mass vascularization