Lee, Byung-Hwan Choi, Sun-Hye Hwang, Sung-Hee Kim, Hyeon-Joong Lee, Sang-Mok Kim, Hyoung-Chun Rhim, Hyewhon Nah, Seung-Yeol 2024-01-20T12:31:46Z 2024-01-20T12:31:46Z 2021-09-05 2013-05 0918-6158 https://pubs.kist.re.kr/handle/201004/128116 Ginsenosides is a low molecular weight substance found in ginseng as one of the active ingredients. Ginsenosides, like other herbal medicines, has a wide range of neuropharmacological actions including neuroprotective effects. The alpha 9 alpha 10 nicotinic acetylcholine receptor is one of numerous nicotinic acetylcholine receptors that exists as a heteropentameric form in auditory hair cells of the cochlea. In this study, we report the effects of ginsenosides on rat alpha 9 alpha 10 nicotinic acetylcholine receptor-mediated ion currents using the two-electrode voltage clamp technique. Treatment with acetylcholine evoked inward currents (I-Ach) in oocytes heterologously expressing the alpha 9 alpha 10 nicotinic acetylcholine receptor. Ginsenosides blocked I-Ach in order of potency of Rg(3)> Rb-2> CK>Re=Rg(2)> Rf>Rc> Rb-1> Rg(1) with reversible manners, and the blocking effect of Rg(3) on I-Ach was same after pre-application than co-application of Rg(3)(-). The half maximal inhibitory concentration (IC50) of Rg(3) was 39.6 +/- 4.9 mu M. Rg(3)-induced I-Ach inhibition was not affected by acetylcholine concentration and was independent of membrane holding potential. Although the inhibitory effect of Rg(3) on I-Ach was abolished in oocytes expressing alpha 9 subunit alone, indicating that the presence of alpha 10 subunit might be required for Rg(3)-induced regulations of alpha 9 alpha 10 nicotinic acetylcholine receptor channel activity. These results indicate that alpha 10 subunit of alpha 9 alpha 10 nicotinic acetylcholine receptor might play an important role in Rg(3)-induced regulation of the alpha 9 alpha 10 nicotinic acetylcholine receptor. English PHARMACEUTICAL SOC JAPAN CENTRAL-NERVOUS-SYSTEM COCHLEAR HAIR-CELLS CHOLINERGIC-RECEPTOR SUBUNIT ALPHA-10 FAMILY Effects of Ginsenoside Rg(3) on alpha 9 alpha 10 Nicotinic Acetylcholine Receptor-Mediated Ion Currents Article 10.1248/bpb.b12-01009 1 BIOLOGICAL & PHARMACEUTICAL BULLETIN, v.36, no.5, pp.812 - 818 BIOLOGICAL & PHARMACEUTICAL BULLETIN 36 5 812 818 scie scopus 000318331400019 2-s2.0-84876921201 Pharmacology & Pharmacy Pharmacology & Pharmacy Article CENTRAL-NERVOUS-SYSTEM COCHLEAR HAIR-CELLS CHOLINERGIC-RECEPTOR SUBUNIT ALPHA-10 FAMILY ginseng ginsenoside alpha 9 alpha 10 nicotinic acetylcholine receptor Xenopus oocyte