Kim, Dong-Eog Kim, Jeong-Yeon Sun, In-Cheol Schellingerhout, Dawid Lee, Su-Kyoung Ahn, Cheol-Hee Kwon, Ick Chan Kim, Kwangmeyung 2024-01-20T12:31:49Z 2024-01-20T12:31:49Z 2021-09-05 2013-05 0364-5134 https://pubs.kist.re.kr/handle/201004/128118 Objective Advancing the understanding and management of thromboembolic stroke requires simple and robust new methods that would be useful for the in vivo assessment of thrombus burden/distribution and for characterizing its evolution in a prompt and quantitative manner. Methods Animals (n=127) with experimental models of thrombosis were imaged with microcomputed tomography 5 minutes (and/or approximate to 3 weeks) after intravenous injection of glycol chitosan (GC) gold nanoparticles (AuNPs). Results Nanoparticles accumulated in the thrombus, allowing computed tomography visualization of both the presence and extent of primary and recurrent thrombi in mouse carotid arteries without a single failure of detection. Nanoparticle thrombus imaging was also effective in monitoring the therapeutic efficacy of thrombolysis (n=118 tissue plasminogen activator [tPA] therapies). Thrombus evolution (either spontaneous or post-tPA) could be mapped at high resolution in both space and time. Due to a long circulating half-life, GC-AuNPs remain available for entrapment into fibrin matrix for an extended period of time (up to 3 weeks), allowing repetition or ongoing monitoring of thrombogenesis and thrombolysis. Interpretation This is the first report on a hyperacute direct thrombus imaging technique using thrombus-seeking AuNPs and computed tomography. When translated into stroke practice, the thrombus imaging may allow us to advance to personalized thrombolytic therapy by demonstrating thrombus burden, distribution, and character in a prompt and quantitative manner. Further study into this area is indicated. ANN NEUROL 2013;73:617-625 English WILEY IN-VIVO CONTRAST AGENT TISSUE FACTOR THROMBOLYSIS STROKE THERAPY BURDEN GROWTH FIBRIN Hyperacute direct thrombus imaging using computed tomography and gold nanoparticles Article 10.1002/ana.23849 1 ANNALS OF NEUROLOGY, v.73, no.5, pp.617 - 625 ANNALS OF NEUROLOGY 73 5 617 625 scie scopus 000319524400010 2-s2.0-84878312414 Clinical Neurology Neurosciences Neurosciences & Neurology Article IN-VIVO CONTRAST AGENT TISSUE FACTOR THROMBOLYSIS STROKE THERAPY BURDEN GROWTH FIBRIN