Kim, Dong-Ju Park, Mee-Rye Lim, Dae-Soon Choi, Jae-Woo 2024-01-20T12:32:01Z 2024-01-20T12:32:01Z 2021-09-04 2013-05 0273-2289 https://pubs.kist.re.kr/handle/201004/128129 In this study, we investigated the impact of nitrate dose on toluene degradation by Pseudomonas putida to elucidate the upper limit of nitrate concentration and whether an optimum ratio of nitrate to toluene concentration exists. Batch microcosm studies were conducted in order to monitor toluene degradation for various ratios (2-20) of nitrate to toluene with nitrate concentrations ranging from 0 to 700 mg L-1 for a given toluene concentration of 50 and 25 mg L-1 during 4-day (short term) and 14-day (long term) incubation time, respectively. The short-term study revealed that nitrate concentration of 500 mg L-1 was toxic to bacteria and the optimum concentration was 300 mg L-1 yielding the highest toluene degradation rate (0.083 mg L-1 h(-1)). In the batch study of long term, toluene degradation was limited to 6 days after which the nitrate at 50 mg L-1 was depleted, indicating that nitrate was a necessary electron acceptor. For both batch studies, an optimum ratio of 6 was found yielding the highest toluene degradation rate. This indicates that an appropriate nitrate dose is essential for efficient degradation of toluene when bioremediation of groundwater contaminated with toluene is under consideration. English HUMANA PRESS INC Impact of Nitrate Dose on Toluene Degradation under Denitrifying Condition Article 10.1007/s12010-013-0176-4 1 APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, v.170, no.2, pp.248 - 256 APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 170 2 248 256 N scie scopus 000317924000002 2-s2.0-84876669833 Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Article GROWTH-KINETICS PSEUDOMONAS-PUTIDA BIODEGRADATION BENZENE INHIBITION CULTURES SULFATE OXYGEN Toluene Degradation Nitrate dose Optimum ratio Pseudomonas putida