Park, Seung-Yoon Kim, In-San 2024-01-20T12:33:04Z 2024-01-20T12:33:04Z 2022-01-10 2013-04 1023-3830 https://pubs.kist.re.kr/handle/201004/128179 A low pH microenvironment is a characteristic feature of inflammation loci and affects the functions of immune cells. In this study, we investigated the effect of extracellular acidification on macrophage gene expression. RAW264.7 macrophages were incubated in neutral (pH 7.4) or acidic (pH 6.8) medium for 4 h. Global mRNA expression levels were determined using Affymetrix genechips. The mRNA expressions of 353 macrophage genes were significantly modified after incubation in acidic medium; 193 were up-regulated and 160 down-regulated. Differentially regulated genes were grouped into 13 classes based on the functions of the corresponding protein products. Pathway analysis revealed that differentially expressed genes are enriched in pathways related to inflammation and immune responses. Quantitative real-time PCR analysis confirmed that the expressions of CXCL10, CXCL14, IL-18, IL-4RA, ABCA1, CCL4, IL-7R, CXCR4, TLR7, and CCL3 mRNAs were regulated by extracellular acidification. The results of this study provide insights into the effects of acidic extracellular environments on macrophage gene expression. English SPRINGER BASEL AG Identification of macrophage genes responsive to extracellular acidification Article 10.1007/s00011-013-0591-6 1 INFLAMMATION RESEARCH, v.62, no.4, pp.399 - 406 INFLAMMATION RESEARCH 62 4 399 406 N scie scopus 000316225200006 2-s2.0-84876694486 Cell Biology Immunology Cell Biology Immunology Article KAPPA-B ACTIVATION APOPTOTIC CELLS ALPHA-V-BETA-5 INTEGRIN DEPENDENT PHAGOCYTOSIS BLOOD-FLOW PH INFLAMMATION NEUTROPHIL EXPRESSION PHOSPHATIDYLSERINE Gene expression Extracellular acidification Macrophages Microarray