Yang, Yoosoo Oh, Jung-Mi Heo, Paul Shin, Jae Yoon Kong, Byoungjae Shin, Jonghyeok Lee, Ji-Chun Oh, Jeong Su Park, Kye Won Lee, Choong Hwan Shin, Yeon-Kyun Kweon, Dae-Hyuk 2024-01-20T12:34:26Z 2024-01-20T12:34:26Z 2021-09-01 2013-03-15 0264-6021 https://pubs.kist.re.kr/handle/201004/128246 Anti-allergic effects of dietary polyphenols were extensively studied in numerous allergic disease models, but the molecular mechanisms of anti-allergic effects by polyphenols remain poorly understood. In the present study, we show that the release of granular cargo molecules, contained in distinct subsets of granules of mast cells, is specifically mediated by two sets of SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins, and that various polyphenols differentially inhibit the formation of those SNARE complexes. Expression analysis of RBL-2H3 cells for 11 SNARE genes and a lipid mixing assay of 24 possible combinations of reconstituted SNAREs indicated that the only two active SNARE complexes involved in mast cell degranulation are Syn (syntaxin) 4/SNAP (23 kDa synaptosome-associated protein)-23/VAMP (vesicle-associated membrane protein) 2 and Syn4/SNAP-23NAMP8. Various polyphenols selectively or commonly interfered with ternary complex formation of these two SNARE complexes, thereby stopping membrane fusion between granules and plasma membrane. This led to the differential effect of polyphenols on degranulation of three distinct subsets of granules. These results suggest the possibility that formation of a variety of SNARE complexes in numerous cell types is controlled by polyphenols which, in turn, might regulate corresponding membrane trafficking. English PORTLAND PRESS LTD FC-EPSILON-RI SECRETORY GRANULES HISTAMINE-RELEASE DIETARY-INTAKE SERUM-ALBUMIN TEA CATECHIN GREEN TEA EXPRESSION EXOCYTOSIS RECEPTOR Polyphenols differentially inhibit degranulation of distinct subsets of vesicles in mast cells by specific interaction with granule-type-dependent SNARE complexes Article 10.1042/BJ20121256 1 BIOCHEMICAL JOURNAL, v.450, pp.537 - 546 BIOCHEMICAL JOURNAL 450 537 546 scie scopus 000315745300010 2-s2.0-84874591436 Biochemistry & Molecular Biology Biochemistry & Molecular Biology Article FC-EPSILON-RI SECRETORY GRANULES HISTAMINE-RELEASE DIETARY-INTAKE SERUM-ALBUMIN TEA CATECHIN GREEN TEA EXPRESSION EXOCYTOSIS RECEPTOR degranulation beta-hexosaminidase histamine membrane fusion polyphenol soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE)