Tsuda, Sachiko Kee, Michelle Z. L. Cunha, Catarina Kim, Jinsook Yan, Ping Loew, Leslie M. Augustine, George J. 2024-01-20T13:04:02Z 2024-01-20T13:04:02Z 2022-01-10 2013-01 0168-0102 https://pubs.kist.re.kr/handle/201004/128471 Recent advances in our understanding of brain function have come from using light to either control or image neuronal activity. Here we describe an approach that combines both techniques: a micromirror array is used to photostimulate populations of presynaptic neurons expressing channelrhodopsin-2, while a red-shifted voltage-sensitive dye allows optical detection of resulting postsynaptic activity. Such technology allowed us to control the activity of cerebellar interneurons while simultaneously recording inhibitory responses in multiple Purkinje neurons, their postsynaptic targets. This approach should substantially accelerate our understanding of information processing by populations of neurons within brain circuits. (C) 2013 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved. English ELSEVIER IRELAND LTD NEURAL ACTIVITY CEREBELLAR CORTEX TRANSGENIC MICE OPTICAL CONTROL INHIBITION INTERNEURONS INDICATOR CHANNELRHODOPSIN-2 INTERROGATION OSCILLATIONS Probing the function of neuronal populations: Combining micromirror-based optogenetic photostimulation with voltage-sensitive dye imaging Article 10.1016/j.neures.2012.11.006 1 NEUROSCIENCE RESEARCH, v.75, no.1, pp.76 - 81 NEUROSCIENCE RESEARCH 75 1 76 81 scie scopus 000316435700013 2-s2.0-84875369070 Neurosciences Neurosciences & Neurology Article NEURAL ACTIVITY CEREBELLAR CORTEX TRANSGENIC MICE OPTICAL CONTROL INHIBITION INTERNEURONS INDICATOR CHANNELRHODOPSIN-2 INTERROGATION OSCILLATIONS Optogenetics Channelrhodopsin Digital micromirror device Voltage-sensitive dye imaging Inhibitory circuitry Cerebellum