Jo, Hyoung Lee, Hee Ju Kim, Chul Young Son, Jin-Ki Jung, Sang Hoon 2024-01-20T13:04:09Z 2024-01-20T13:04:09Z 2021-09-01 2013-01 0278-6915 https://pubs.kist.re.kr/handle/201004/128478 Retinal ganglion cells (RGCs) death caused by oxidative stress is a common risk factor for glaucoma. In the present study, 8-hydroxycalamenene was isolated from the hexane fraction of Reynoutria elliptica. We showed that 8-hydroxycalamenene attenuated the cell death of transformed RGC-5 cells. This compound also produced a dose-dependent decrease in the expression of apoptotic proteins (cleaved PARP and caspase-3) induced by L-buthionine-(S,R)-sulfoximine (BSO) plus glutamate and stimulated glutathione and glutathione S-transferase activity. Moreover, the addition of 8-hydroxycalamenene to cell cultures restored the reduced mitochondrial membrane potential resulting from glutamate/BSO treatment. The presence of N-methyl-D-aspartate in the retina of rats affected the thickness of the inner plexiform layer (IPL) and increased the number of TUNEL-positive RGCs. However, 8-hydroxycalamenene protected against thinning of the IPL and reduced TUNEL-positive cells in the ganglion cell layer. Thus, 8-hydroxycalamenene isolated from R. elliptica exerts neuroprotective effects both in vitro and in vivo. (C) 2012 Elsevier Ltd. All rights reserved. English PERGAMON-ELSEVIER SCIENCE LTD RETINAL GANGLION-CELLS OXIDATIVE STRESS GLAUCOMA LINE MECHANISM DEATH EXCITOTOXICITY ANTIOXIDANT MODULATION INHIBITORS 8-Hydroxycalamenene isolated from the rhizomes of Reynoutria elliptica exerts neuroprotective effects both in vitro and in vivo Article 10.1016/j.fct.2012.09.025 1 FOOD AND CHEMICAL TOXICOLOGY, v.51, pp.231 - 241 FOOD AND CHEMICAL TOXICOLOGY 51 231 241 scie scopus 000315078300030 2-s2.0-84867896916 Food Science & Technology Toxicology Food Science & Technology Toxicology Article RETINAL GANGLION-CELLS OXIDATIVE STRESS GLAUCOMA LINE MECHANISM DEATH EXCITOTOXICITY ANTIOXIDANT MODULATION INHIBITORS Reynoutria elliptica 8-Hydroxycalamenene Neuroprotection RGC-5