Jin, Ming J. Lee, Jaeick In, Moon K. Yoo, Hye H. 2024-01-20T13:04:17Z 2024-01-20T13:04:17Z 2021-09-01 2013-01 0022-1198 https://pubs.kist.re.kr/handle/201004/128485 CP 47,497, a potent cannabinoid receptor type 1 agonist, is the main active ingredient in the herbal mixture Spice sold in European countries. The illegal use of Spice for its psychoactive effects has become a social issue. In this study, the in vitro metabolism of CP 47,497 was investigated in human liver microsomes to characterize the metabolic fate of CP 47,497. CP 47,497 was incubated with human liver microsomes, and the reaction mixture was analyzed using liquid chromatography-tandem mass spectrometry. A total of eight metabolites were detected in human liver microsomes and structurally characterized based on mass spectral data. The main metabolic pathways involved hydroxylations or oxygenations. The identified metabolites were mono-oxygenated metabolites (M1 and M4), mono-hydroxylated metabolites (M3, M5, M6, M7, and M8), and a di-oxygenated metabolite (M2). The detection of these metabolites could confirm the presence of CP 47,497 in biological samples; therefore, collectively, they would be excellent indicators of Spice drug abuse. English WILEY-BLACKWELL JWH-018 URINE Characterization of In Vitro Metabolites of CP 47,497, a Synthetic Cannabinoid, in Human Liver Microsomes by LC-MS/MS Article 10.1111/j.1556-4029.2012.02261.x 1 JOURNAL OF FORENSIC SCIENCES, v.58, no.1, pp.195 - 199 JOURNAL OF FORENSIC SCIENCES 58 1 195 199 scie scopus 000313709500031 2-s2.0-84872417570 Medicine, Legal Legal Medicine Article JWH-018 URINE forensic science spice CP 47 497 metabolism LC-MS MS