Park, Mi-Ran Seo, Bo-Bae Song, Soo-Chang 2024-01-20T13:04:28Z 2024-01-20T13:04:28Z 2021-09-01 2013-01 0142-9612 https://pubs.kist.re.kr/handle/201004/128494 A dual ionic interaction system composed of a positively charged polyelectrolyte complex (PEC) containing human growth hormone (hGH) and anionic thermosensitive hydrogel has been suggested for sustained delivery of bioactive hGH. The PEC was prepared by ionic interaction between negatively charged hGH and positively charged protamine sulfate (PS) to suppress diffusion of hGH. Moreover, we loaded the positively charged PEC into an anionic, injectable, and thermosensitive poly(organophosphazene) hydrogel to enhance sustained release of hGH by dual ionic interactions. PS formed a spherical complex with hGH, and their ionic interaction grew stronger with increasing amounts of PS. From a weight ratio of 0.5, the PS/hGH complex had a size and zeta-potential that were constantly maintained around 500 nm and +8 mV, respectively, in 0.9% NaCl. The PEC-loaded hydrogels suppressed the initial burst release of hGH and extended the release period in vitro and in vivo. In a pharmacokinetic study in rats, the PEC-loaded anionic hydrogel extended half-life 13-fold with similar area under the curve (AUC) compared to hGH solution. Furthermore, single injection of PEC-loaded anionic hydrogel showed a more increased growth rate than daily injection of hGH solution for 7 days in hypophysectomized rats, demonstrating its potential as an injectable, sustained delivery system that can release bioactive hGH. (C) 2012 Elsevier Ltd. All rights reserved. English ELSEVIER SCI LTD CONTROLLED DELIVERY SYSTEMS BIOMEDICAL APPLICATIONS PROTEIN DELIVERY DRUG-DELIVERY MICROSPHERES FORMULATION POLYPHOSPHAZENES CHALLENGES CHITOSAN Dual ionic interaction system based on polyelectrolyte complex and ionic, injectable, and thermosensitive hydrogel for sustained release of human growth hormone Article 10.1016/j.biomaterials.2012.10.033 1 BIOMATERIALS, v.34, no.4, pp.1327 - 1336 BIOMATERIALS 34 4 1327 1336 scie scopus 000313155800045 2-s2.0-84870365758 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article CONTROLLED DELIVERY SYSTEMS BIOMEDICAL APPLICATIONS PROTEIN DELIVERY DRUG-DELIVERY MICROSPHERES FORMULATION POLYPHOSPHAZENES CHALLENGES CHITOSAN Human growth hormone (hGH) Protamine sulfate (PS) Polyelectrolyte complex (PEC) Anionic, injectable and thermosensitive hydrogel Poly(organophosphazene) Sustained release