Fukui, Masayuki Kang, Ki Sung Okada, Kazushi Zhu, Bao Ting 2024-01-20T13:30:19Z 2024-01-20T13:30:19Z 2021-08-31 2013-01 0730-2312 https://pubs.kist.re.kr/handle/201004/128533 In a recent study, we showed that eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), two common omega-3 fatty acids, can cause ROS accumulation and subsequently induce caspase-8-dependent apoptosis in human breast cancer cells (Kang et al. [2010], PLoS ONE 5: e10296). In this study, we showed that the pancreas has a unique ability to accumulate EPA at a level markedly higher than several other tissues analyzed. Based on this finding, we sought to further investigate the anticancer actions of EPA and its analog DHA in human pancreatic cancer cells using both in vitro and in vivo models. EPA and DHA were found to induce ROS accumulation and caspase-8-dependent cell death in human pancreatic cancer cells (MIA-PaCa-2 and Capan-2) in vitro. Feeding animals with a diet supplemented with 5% fish oil, which contains high levels of EPA and DHA, also strongly suppresses the growth of MIA-PaCa-2 human pancreatic cancer xenografts in athymic nude mice, by inducing oxidative stress and cell death. In addition, we showed that EPA can concomitantly induce autophagy in these cancer cells, and the induction of autophagy diminishes its ability to induce apoptotic cell death. It is therefore suggested that combination of EPA with an autophagy inhibitor may be a useful strategy in increasing the therapeutic effectiveness in pancreatic cancer. J. Cell. Biochem. 114: 192203, 2012. (C) 2012 Wiley Periodicals, Inc. English WILEY POLYUNSATURATED FATTY-ACIDS FISH-OIL DOCOSAHEXAENOIC ACID LIPID-PEROXIDATION LINOLENIC ACID DEATH PROLIFERATION GROWTH YOUNG SUPPLEMENTATION EPA, an omega-3 fatty acid, induces apoptosis in human pancreatic cancer cells: Role of ROS accumulation, caspase-8 activation, and autophagy induction Article 10.1002/jcb.24354 1 JOURNAL OF CELLULAR BIOCHEMISTRY, v.114, no.1, pp.192 - 203 JOURNAL OF CELLULAR BIOCHEMISTRY 114 1 192 203 scie scopus 000311112700021 2-s2.0-84869392417 Biochemistry & Molecular Biology Cell Biology Biochemistry & Molecular Biology Cell Biology Article POLYUNSATURATED FATTY-ACIDS FISH-OIL DOCOSAHEXAENOIC ACID LIPID-PEROXIDATION LINOLENIC ACID DEATH PROLIFERATION GROWTH YOUNG SUPPLEMENTATION EICOSAPENTAENOIC ACID DOCOSAHEXAENOIC ACID OMEGA-3 POLYUNSATURATED FATTY ACIDS PANCREATIC CANCER APOPTOSIS AUTOPHAGY