Kim, Jung-Min Shin, Hong-In Cha, Sun-Shin Lee, Chang Sup Hong, Bok Sil Lim, Seyoung Jang, Hyun-Jun Kim, Jaeyoon Yang, Yong Ryoul Kim, Yun-Hee Yun, Sanguk Rijal, Girdhari Lee-Kwon, Whaseon Seo, Jeong Kon Gho, Yong Song Ryu, Sung Ho Hur, Eun-Mi Suh, Pann-Ghill 2024-01-20T13:31:23Z 2024-01-20T13:31:23Z 2021-09-05 2012-12 2041-1723 https://pubs.kist.re.kr/handle/201004/128586 Communication between osteoblasts and endothelial cells is essential for bone fracture repair, but the molecular identities of such communicating factors are not well defined. Here we identify DJ-1 as a novel mediator of the cross-talk between osteoblasts and endothelial cells through an unbiased screening of molecules secreted from human mesenchymal stem cells during osteogenesis. We show that DJ-1 stimulates the differentiation of human mesenchymal stem cells to osteoblasts and that DJ-1 induces angiogenesis in endothelial cells through activation of fibroblast growth factor receptor-1 signalling. In a rodent model of bone fracture repair, extracellular application of DJ-1 enhances bone regeneration in vivo by stimulating the formation of blood vessels and new bones. Both these effects are blocked by antagonizing fibroblast growth factor receptor-1 signalling. These findings uncover previously undefined extracellular roles of DJ-1 to promote angiogenesis and osteogenesis, suggesting DJ-1 may have therapeutic potential to stimulate bone regeneration. English NATURE PUBLISHING GROUP ENDOTHELIAL-GROWTH-FACTOR BONE-FORMATION PARKINSONS-DISEASE PROTEIN-KINASE CELLS VEGF EXPRESSION PATHWAY DEFECT IDENTIFICATION DJ-1 promotes angiogenesis and osteogenesis by activating FGF receptor-1 signaling Article 10.1038/ncomms2313 1 NATURE COMMUNICATIONS, v.3 NATURE COMMUNICATIONS 3 scie scopus 000316356700063 2-s2.0-84871775091 Multidisciplinary Sciences Science & Technology - Other Topics Article ENDOTHELIAL-GROWTH-FACTOR BONE-FORMATION PARKINSONS-DISEASE PROTEIN-KINASE CELLS VEGF EXPRESSION PATHWAY DEFECT IDENTIFICATION Signaling