Park, Gi Cheol Lee, Miji Roh, Jong-Lyel Yu, Myeong Sang Choi, Seung-Ho Nam, Soon Yuhl Kim, Sang Yoon Cho, Kyung-Ja 2024-01-20T13:31:52Z 2024-01-20T13:31:52Z 2021-09-05 2012-12 1368-8375 https://pubs.kist.re.kr/handle/201004/128610 Objectives: Human papillomavirus (HPV), a causative agent of oropharyngeal squamous cell carcinoma (SCC), may be detected in metastatic cervical lymph nodes (MCNs). We investigated whether HPV and p16 expression in MCNs can help identify oropharyngeal primaries in patients with cervical lymph node metastases from an unknown primary tumor (CUP) and predict their survival outcomes. Materials and methods: SCC MCNs of 58 patients with CUP were assayed for HPV by in situ hybridization and for p16 and p53 expression by immunohistochemistry. The presence of HPV and p16 in MCN was correlated with oropharyngeal tumor location and prognosis. Results: Oropharyngeal primaries were found in 20 patients. The sensitivity and negative predictive value (NPV) of HPV for oropharyngeal localization were 90.0% and 92.6%, respectively, and the sensitivity and NPV of p16 were 80.0% and 86.2%, respectively. Multivariate analyses showed that the location of the largest MCN (P = 0.035) and HPV (P = 0.004) were independent predictors of oropharyngeal tumors. Multivariate analyses showed that p16 expression was an independent predictor of disease-free survival (P = 0.030; hazard ratio (HR) = 0.286; 95% CI, 0.092-0.887) and that p53 expression (P = 0.017; HR = 3.154, 95% CI = 1.288-8.103) and extracapsular extension of MCN (P = 0.010; HR = 3.924, 95% CI = 1.387-11.097) were independent predictors of overall survival. Conclusion: Detection of HPV and p16 may help identify hidden oropharyngeal primaries in CUP patients and predict their survival outcomes. (C) 2012 Elsevier Ltd. All rights reserved. English ELSEVIER SCIENCE BV SQUAMOUS-CELL CARCINOMA NECK-CANCER OROPHARYNGEAL CANCER GENE-PRODUCT HPV-DNA HEAD EXPRESSION PROGNOSIS P53 SURVIVAL Human papillomavirus and p16 detection in cervical lymph node metastases from an unknown primary tumor Article 10.1016/j.oraloncology.2012.05.026 1 ORAL ONCOLOGY, v.48, no.12, pp.1250 - 1256 ORAL ONCOLOGY 48 12 1250 1256 scie scopus 000311151200009 2-s2.0-84869499953 Oncology Dentistry, Oral Surgery & Medicine Oncology Dentistry, Oral Surgery & Medicine Article SQUAMOUS-CELL CARCINOMA NECK-CANCER OROPHARYNGEAL CANCER GENE-PRODUCT HPV-DNA HEAD EXPRESSION PROGNOSIS P53 SURVIVAL Human papillomavirus p16 Cervical lymph node metastases from an unknown primary tumor Primary tumor location Survivals