Lee, H.S. Yoon, J.-S. Song, M. Shin, C.-Y. Chung, H.S. Ryu, J.-C. 2024-01-20T13:32:25Z 2024-01-20T13:32:25Z 2021-08-31 2012-12 2005-9752 https://pubs.kist.re.kr/handle/201004/128635 Low-molecular weight saturated aliphatic aldehydes (LSAAs), which include propanal, butanal, pentanal, hexanal, octanal, nonanal and heptanal, are volatile organic compounds (VOCs). They are ubiquitous in the environment of our daily life. Although LSAAs are harmful, with mutagenic and carcinogenic effects, the mechanisms underlying the toxicity of volatile aldehydes are still unclear. Therefore, in this study, we performed genome-wide expression profile analysis of A549 human alveolar epithelial cells exposed to seven LSSAs. We selected genes whose expression was changed more than 1.5-fold in A549 cells exposed to LSAAs by analysis of gene expression profiles using human oligonucleotide chips. Through gene expression profiling, we showed that LSSAs are related to the key biological processes defense response, inflammatory response and immune response in gene ontology (GO) analysis. In addition, we identified two genes that were up-regulated (GREB1, BC009808) and four that were down-regulated (UCP1, TCP11, FNDC3A, LOC645206) by all the tested LSAAs. Our data suggest that LSAAs exert toxic effects on A549 cells by modulating mRNA expression. Moreover, we suggest that genes expressed in response to LSAAs represent a molecular signature that can be widely used, in combination with more traditional techniques, to assess and predict the toxicity caused by exposure to LSAAs. ？ 2012 Korean Society of Environmental Risk Assessment and Health Science and Springer Science+Business Media Dordrecht. English Kluwer Academic Publishers 3 (4, 5 dimethyl 2 thiazolyl) 2, 5 diphenyltetrazolium bromide aldehyde butyraldehyde estrogen heptanaldehyde hexanal messenger RNA nonanaldehyde octanal pentanaldehyde propionaldehyde unclassified drug air pollutant article cancer cell cancer cell culture cell adhesion cell hybridization cell proliferation cell viability controlled study cytotoxicity down regulation environmental exposure gene expression genetic analysis genome analysis human human cell immune response in vitro study inflammation lung alveolus epithelium lung cancer microarray analysis nucleotide sequence spectrophotometry upregulation Gene expression profiling of low dose exposure of saturated aliphatic aldehydes in A549 human alveolar epithelial cells Article 10.1007/s13530-012-0140-7 1 Toxicology and Environmental Health Sciences, v.4, no.4, pp.211 - 217 Toxicology and Environmental Health Sciences 4 4 211 217 scopus 2-s2.0-84872356063 Article 3 (4, 5 dimethyl 2 thiazolyl) 2, 5 diphenyltetrazolium bromide aldehyde butyraldehyde estrogen heptanaldehyde hexanal messenger RNA nonanaldehyde octanal pentanaldehyde propionaldehyde unclassified drug air pollutant article cancer cell cancer cell culture cell adhesion cell hybridization cell proliferation cell viability controlled study cytotoxicity down regulation environmental exposure gene expression genetic analysis genome analysis human human cell immune response in vitro study inflammation lung alveolus epithelium lung cancer microarray analysis nucleotide sequence spectrophotometry upregulation A549 Aldehydes Gene ontology (GO) Microarray