Jun, Seung-Hyun Kim, Kwanghee An, Hyo Jin Kim, Byoung Chan Sonn, Chung Hee Kim, Miju Doh, Junsang Yee, Cassian Lee, Kyung-Mi Kim, Jungbae 2024-01-20T13:32:52Z 2024-01-20T13:32:52Z 2021-09-05 2012-11-07 1616-301X https://pubs.kist.re.kr/handle/201004/128656 A novel cell isolation and release platform using electrospun polystyrene-poly(styrene-co-maleic anhydride) (PS-PSMA) nanofibers is presented. Ethanol treatment of PS-PSMA nanofibers, employed for the purpose of sterilization, significantly increases their inter-fiber space for both antibody conjugation and subsequent cell separation. For the selective isolation of CD4(+) T cells from heterogeneous mixtures of mouse lymph nodes, capture efficiencies of up to 100% are achieved while maintaining cellular integrity and viability. Once captured, CD4(+) T lymphocytes can also be released from the NF scaffolds, further demonstrating its potential functionality as an immune cell-supporting and releasing matrix. This is the first demonstration of lymphocyte-culture scaffolds enabling selective isolation, accommodation, and sustained release of CD4(+) T cells for the purpose of cell therapies. English WILEY-V C H VERLAG GMBH PANCREATIC-CANCER PROTEIN ELECTROSPUN DELIVERY COATINGS HER2/NEU Ethanol-Dispersed Polymer Nanofibers as a Highly Selective Cell Isolation and Release Platform for CD4(+) T Lymphocytes Article 10.1002/adfm.201200657 1 ADVANCED FUNCTIONAL MATERIALS, v.22, no.21, pp.4448 - 4455 ADVANCED FUNCTIONAL MATERIALS 22 21 4448 4455 scie scopus 000310602700006 2-s2.0-84868537746 Chemistry, Multidisciplinary Chemistry, Physical Nanoscience & Nanotechnology Materials Science, Multidisciplinary Physics, Applied Physics, Condensed Matter Chemistry Science & Technology - Other Topics Materials Science Physics Article PANCREATIC-CANCER PROTEIN ELECTROSPUN DELIVERY COATINGS HER2/NEU electrospun polymer nanofibers ethanol dispersion CD4+T lymphocyte cell separation cell transplantation