Oh, Soo-Jin Han, Kyung-Seok Park, Hyungju Woo, Dong Ho Kim, Hye Yun Traynelis, Stephen F. Lee, C. Justin 2024-01-20T13:35:02Z 2024-01-20T13:35:02Z 2021-09-05 2012-10-12 1756-6606 https://pubs.kist.re.kr/handle/201004/128765 Background: Glutamate is the major transmitter that mediates the principal form of excitatory synaptic transmission in the brain. It has been well established that glutamate is released via Ca2+-dependent exocytosis of glutamate-containing vesicles in neurons. However, whether astrocytes exocytose to release glutamate under physiological condition is still unclear. Findings: We report a novel form of glutamate release in astrocytes via the recently characterized Ca2+-activated anion channel, Bestrophin-1 (Best1) by Ca2+ dependent mechanism through the channel pore. We demonstrate that upon activation of protease activated receptor 1 (PAR1), an increase in intracellular Ca2+ concentration leads to an opening of Best1 channels and subsequent release of glutamate in cultured astrocytes. Conclusions: These results provide strong molecular evidence for potential astrocyte-neuron interaction via Best1-mediated glutamate release. English BMC SYNAPTIC-TRANSMISSION TRANSMITTER RELEASE HIPPOCAMPAL-NEURONS BEST-DISEASE D-ASPARTATE D-SERINE CALCIUM CA2+ L-TRANS-PYRROLIDINE-2,4-DICARBOXYLATE POTENTIATION Protease activated receptor 1-induced glutamate release in cultured astrocytes is mediated by Bestrophin-1 channel but not by vesicular exocytosis Article 10.1186/1756-6606-5-38 1 MOLECULAR BRAIN, v.5 MOLECULAR BRAIN 5 scie scopus 000313275300001 2-s2.0-84867318753 Neurosciences Neurosciences & Neurology Article SYNAPTIC-TRANSMISSION TRANSMITTER RELEASE HIPPOCAMPAL-NEURONS BEST-DISEASE D-ASPARTATE D-SERINE CALCIUM CA2+ L-TRANS-PYRROLIDINE-2,4-DICARBOXYLATE POTENTIATION Astrocyte Bestrophin-1 Glutamate