Kim, Y. H. Park, S. Y. Park, J. Kim, Y. S. Hwang, E. M. Park, J. Y. Roh, G. S. Kim, H. J. Kang, S. S. Cho, G. J. Choi, W. S. 2024-01-20T14:01:14Z 2024-01-20T14:01:14Z 2021-09-05 2012-10 0012-186X https://pubs.kist.re.kr/handle/201004/128823 The study aimed to evaluate the efficacy of recombinant adenovirus expressing alpha A-crystallin (Ad-alpha Ac-Gfp) in reducing pericyte loss within retinal vasculature in early diabetes. Diabetes was induced by streptozotocin injection into C57BL/6 mice. Ad-alpha Ac-Gfp was delivered by intravitreous injection to the right eyes of mice 2 weeks before induction of diabetes. Vascular leakage was determined by fluorescent angiography, Evans Blue leakage assay and leucocyte adhesion test. Production of alpha A-crystallin was analysed by immunoblotting and double immunostaining and pericyte loss was analysed by pericyte count. Vessel leakage and pericyte loss were observed in the streptozotocin-induced diabetic retina. Decreased abundance of alpha A-crystallin in retinas 2 and 6 months after the induction of diabetes was confirmed by two-dimensional electrophoretic analysis, immunoblotting and RT-PCR. Double immunofluorescence staining for alpha A-crystallin and NG2 chondroitin sulphate proteoglycan revealed that alpha A-crystallin was predominantly produced in the retinal pericyte and that the number of alpha A-crystallin-producing pericytes decreased in the diabetic retina. Retinal infection with Ad-alpha Ac-Gfp led to decreased pericyte loss and vascular leakage compared with control. Intravitreal delivery of Ad-alpha Ac-Gfp protects against vascular leakage in the streptozotocin-induced model of diabetes. This effect is associated with the inhibition of diabetic retinal pericyte loss in early diabetes, suggesting that alpha A-crystallin has a role in preventing the pathogenesis of early diabetic retinopathy. English SPRINGER RETINAL BARRIER BREAKDOWN HEAT-SHOCK-PROTEIN B-CRYSTALLIN CHAPERONE FUNCTION CELL GROWTH BLOOD-FLOW RETINOPATHY EXPRESSION MOUSE VEGF Reduction of experimental diabetic vascular leakage and pericyte apoptosis in mice by delivery of alpha A-crystallin with a recombinant adenovirus Article 10.1007/s00125-012-2625-y 1 DIABETOLOGIA, v.55, no.10, pp.2835 - 2844 DIABETOLOGIA 55 10 2835 2844 scie scopus 000308354600034 2-s2.0-84866083771 Endocrinology & Metabolism Endocrinology & Metabolism Article RETINAL BARRIER BREAKDOWN HEAT-SHOCK-PROTEIN B-CRYSTALLIN CHAPERONE FUNCTION CELL GROWTH BLOOD-FLOW RETINOPATHY EXPRESSION MOUSE VEGF alpha A-Crystallin BRB breakdown Diabetic retinopathy Pericyte loss