Rahman, Siti Fauziyah Gobikrishnan, Sriramulu Indrawan, Natarianto Park, Seok-Hwan Park, Jae-Hee Min, Kyoungseon Yoo, Young Je Park, Don-Hee 2024-01-20T14:01:42Z 2024-01-20T14:01:42Z 2021-09-04 2012-10 1017-7825 https://pubs.kist.re.kr/handle/201004/128848 Levodopa or L-3,4-dihydroxyphenylalanine (L-DOPA) is the precursor of the neurotransmitter dopamine. L-DOPA is a famous treatment for Parkinson's disease symptoms. In this study, electroenzymatic synthesis of L-DOPA was performed in a three-electrode cell, comprising a Ag/AgCl reference electrode, a platinum wire auxiliary electrode, and a glassy carbon working electrode. L-DOPA had an oxidation peak at 376 mV and a reduction peak at -550 mV. The optimum conditions of pH, temperature, and amount of free tyrosinase enzyme were pH 7, 30 degrees C, and 250 IU, respectively. The kinetic constant of the free tyrosinase enzyme was found for both cresolase and catacholase activity to be 0.25 and 0.4 mM, respectively. A cyclic voltammogram was used to investigate the electron transfer rate constant. The mean heterogeneous electron transfer rate (k(c)) was 5.8 x 10(-4) cm/s. The results suggest that the electroenzymatic method could be an alternative way to produce L-DOPA without the use of a reducing agent such as ascorbic acid. English KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY TYROSINASE ACID A Study on the Electrochemical Synthesis of L-DOPA Using Oxidoreductase Enzymes: Optimization of an Electrochemical Process Article 10.4014/jmb.1206.06043 1 JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.22, no.10, pp.1446 - 1451 JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY 22 10 1446 1451 scie scopus kci 000310481400019 2-s2.0-84930482656 Biotechnology & Applied Microbiology Microbiology Biotechnology & Applied Microbiology Microbiology Article TYROSINASE ACID L-DOPA electrochemical synthesis tyrosinase Parkinson&apos s disease