Romanova, Elena V. Lee, Ji Eun Kelleher, Neil L. Sweedler, Jonathan V. Gulley, Joshua M. 2024-01-20T14:01:52Z 2024-01-20T14:01:52Z 2021-08-31 2012-10 0022-3042 https://pubs.kist.re.kr/handle/201004/128855 Repeated exposure to amphetamine (AMPH) induces long-lasting behavioral changes, referred to as sensitization, that are accompanied by various neuroadaptations in the brain. To investigate the chemical changes that occur during behavioral sensitization, we applied a comparative proteomics approach to screen for neuropeptide changes in a rodent model of AMPH-induced sensitization. By measuring peptide profiles with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and comparing signal intensities using principal component analysis and variance statistics, subsets of peptides are found with significant differences in the dorsal striatum, nucleus accumbens, and medial prefrontal cortex of AMPH-sensitized male SpragueDawley rats. These biomarker peptides, identified in follow-up analyses using liquid chromatography and tandem mass spectrometry, suggest that behavioral sensitization to AMPH is associated with complex chemical adaptations that regulate energy/metabolism, neurotransmission, apoptosis, neuroprotection, and neuritogenesis, as well as cytoskeleton integrity and neuronal morphology. Our data contribute to a growing number of reports showing that in addition to the mesolimbic dopamine system, which is the best known signaling pathway involved with reinforcing the effect of psychostimulants, concomitant chemical changes in other pathways and in neuronal organization may play a part in the overall effect of chronic AMPH exposure on behavior. English WILEY MASS-SPECTROMETRY NUCLEUS-ACCUMBENS PREFRONTAL CORTEX FRONTAL-CORTEX MITOCHONDRIAL DYSFUNCTION LIQUID-CHROMATOGRAPHY SENSITIZATION COCAINE IDENTIFICATION PEPTIDES Comparative peptidomics analysis of neural adaptations in rats repeatedly exposed to amphetamine Article 10.1111/j.1471-4159.2012.07912.x 1 JOURNAL OF NEUROCHEMISTRY, v.123, no.2, pp.276 - 287 JOURNAL OF NEUROCHEMISTRY 123 2 276 287 scie scopus 000309454300007 2-s2.0-84867064628 Biochemistry & Molecular Biology Neurosciences Biochemistry & Molecular Biology Neurosciences & Neurology Article MASS-SPECTROMETRY NUCLEUS-ACCUMBENS PREFRONTAL CORTEX FRONTAL-CORTEX MITOCHONDRIAL DYSFUNCTION LIQUID-CHROMATOGRAPHY SENSITIZATION COCAINE IDENTIFICATION PEPTIDES addiction mass spectrometry neurochemical adaptation peptidomics principal component analysis proteo-mics