Park, Kyeong Soon Kang, Jin Hee Sa, Keum Hee Koo, Hee Beom Cho, Hyun Jung Nam, Eon Jeong Youn, In Chan Kim, Kwang Meyung Kim, In San Kwon, Ick Chan Choi, Kui Won Kang, Young Mo 2024-01-20T14:03:32Z 2024-01-20T14:03:32Z 2021-09-04 2012-09 1535-3508 https://pubs.kist.re.kr/handle/201004/128943 We demonstrated that arthritis could be visualized noninvasively using hydrophobically modified glycol chitosan nanoparticles labeled with Cy5.5 (HGC-Cy5.5) and an optical imaging system. Activated macrophages expressing Mac-1 molecules effectively phagocytosed HGC-Cy5.5, which formed spherical nanoparticles under physiologic conditions. We estimated the applicability of HGC-Cy5.5 to quantitative analysis of arthritis development and progression. Near-infrared fluorescence images, captured after HGC-Cy5.5 injection in mice with collagen-induced arthritis, showed stronger fluorescence intensity in the active arthritis group than in the nonarthritis group. According to the progression of arthritis in both collagen-induced arthritis and collagen antibody-induced arthritis models, total photon counts (TPCs) increased in parallel with the clinical arthritis index. Quantitative analysis of fluorescence after treatment with methotrexate showed a significant decrease in TPC in a dose-dependent manner. Histologic evaluation confirmed that the mechanism underlying selective accumulation of HGC-Cy5.5 within synovitis tissues included enhanced phagocytosis of the probe by Mac-1-expressing macrophages as well as enhanced permeability through leaky vessels. These results suggest that optical imaging of arthritis using HGC-Cy5.5 can provide an objective measurement of disease activity and, at the same time, therapeutic responses in rheumatoid arthritis. English SAGE PUBLICATIONS INC SELF-ASSEMBLED NANOPARTICLES RHEUMATOID-ARTHRITIS SYNOVIAL TISSUE ANGIOGENESIS DISEASE CANCER CELLS MICE In Vivo Quantitative Measurement of Arthritis Activity Based on Hydrophobically Modified Glycol Chitosan in Inflammatory Arthritis: More Active than Passive Accumulation Article 10.2310/7290.2011.00056 1 MOLECULAR IMAGING, v.11, no.5, pp.389 - 400 MOLECULAR IMAGING 11 5 389 400 scie scopus 000324579900005 2-s2.0-84873923550 Biochemical Research Methods Radiology, Nuclear Medicine & Medical Imaging Biochemistry & Molecular Biology Radiology, Nuclear Medicine & Medical Imaging Article SELF-ASSEMBLED NANOPARTICLES RHEUMATOID-ARTHRITIS SYNOVIAL TISSUE ANGIOGENESIS DISEASE CANCER CELLS MICE