Cho, Hyun-Jong Yoon, Hong Yeol Koo, Heebeom Ko, Seung-Hak Shim, Jae-Seong Cho, Jee-Hyun Park, Jae Hyung Kim, Kwangmeyung Kwon, Ick Chan Kim, Dae-Duk 2024-01-20T14:04:05Z 2024-01-20T14:04:05Z 2021-09-04 2012-08-20 0168-3659 https://pubs.kist.re.kr/handle/201004/128968 Hyaluronic acid-ceramide (HACE)-based nanoprobes for magnetic resonance (MR) and optical imaging were developed for cancer diagnosis. Diethylenetriaminepentaacetic dianhydride (DTPA) was conjugated to HACE for the chelation of gadolinium (Gd) as an MR contrast agent. Cy5.5 was also conjugated to the HACE backbone as a near-infrared fluorescence (NIRF) imaging dye. The self-assembled HACE-based nanoprobe, Cy5.5-HACE-DTPA-Gd, exhibited a uniformly distributed particle size and morphological shape. The HACE-based nanoprobe did not induce serious cytotoxicity in U87-MG (low expression of CD44 receptor) and SCC7 (high expression of CD44 receptor) cells. The cellular uptake efficiency of the HACE-based nanoprobe was higher in SCC7 cells than in U87-MG cells, indicating an HA-CD44 receptor interaction. In vitro MR signal enhancement of the HACE-based nanoprobe was confirmed compared with a commercial formulation (Magnevist). Moreover, in vivo MR contrast enhancement of the HACE-based nanoprobe in the tumor region was verified in an SCC7 tumor xenograft mouse model. The tumor targetability of the developed nanoprobe was monitored by an NIRF imaging study, and improved accumulation of the nanoprobe in the tumor region was observed. Therefore, this HACE-based dual-imaging nanoprobe can be used to make a more accurate diagnosis of cancer based on its passive and active tumor targeting strategies. (C) 2012 Elsevier B. V. All rights reserved. English ELSEVIER MRI CONTRAST AGENTS SELF-ASSEMBLED NANOPARTICLES MAGNETIC-RESONANCE TARGETED DELIVERY FLUORESCENCE Hyaluronic acid-ceramide-based optical/MR dual imaging nanoprobe for cancer diagnosis Article 10.1016/j.jconrel.2012.06.011 1 JOURNAL OF CONTROLLED RELEASE, v.162, no.1, pp.111 - 118 JOURNAL OF CONTROLLED RELEASE 162 1 111 118 scie scopus 000307769200013 2-s2.0-84864663396 Chemistry, Multidisciplinary Pharmacology & Pharmacy Chemistry Pharmacology & Pharmacy Article MRI CONTRAST AGENTS SELF-ASSEMBLED NANOPARTICLES MAGNETIC-RESONANCE TARGETED DELIVERY FLUORESCENCE Hyaluronic acid-ceramide MRI NIRF Dual modality Tumor targeting