Song, Mee Kim, Youn-Jung Park, Yong-Keun Ryu, Jae-Chun 2024-01-20T14:05:03Z 2024-01-20T14:05:03Z 2021-09-05 2012-08 1464-0325 https://pubs.kist.re.kr/handle/201004/129016 Thyroperoxidase (TPO) is a large heme-containing glycoprotein that catalyzes the transfer of iodine to thyroglobulin during thyroid hormone (TH) synthesis. Previously, we established an in vitro assay for TPO activity based on human recombinant TPO (hrTPO) stably transfected into human follicular thyroid carcinoma (FTC-238) cells. It is important to determine whether environmental chemicals can disrupt TPO activity because it is an important factor in the TH axis. In this study, we used our assay to examine the changes in TPO activity in response to various chemicals, including benzophenones (BPs), polycyclic aromatic hydrocarbons (PAHs), and persistent organic pollutants (POPs). Overall, BPs, PAHs, and POPs slightly altered TPO activity at low doses, as compared with the positive controls methimazole (MMI), genistein, and 2,2',4,4'-tetrahydroxy BP. Benzophenone, benzhydrol, 3-methylchloranthracene, pyrene, benzo(k)fluoranthene, benzo(e)pyrene, perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA), and heptachlor decreased TPO activity, while 2,4-dihydroxy BP, 2,2'-dihydroxy-4-methoxy BP, and dibenzo(a, h) anthracene increased TPO activity. From these data, we can predict the disruption of TPO activity by various chemicals as a sensitive TH end point. TPO activity should be considered when enacting measures to regulate environmental exposure to thyroid-disrupting chemicals. English ROYAL SOC CHEMISTRY IN-VIVO ANALYSIS ENVIRONMENTAL CHEMICALS HORMONE SYNTHESIS TYROSINE MECHANISM VITRO RATS IDENTIFICATION THIOCYANATE IODINATION Changes in thyroid peroxidase activity in response to various chemicals Article 10.1039/c2em30106g 1 JOURNAL OF ENVIRONMENTAL MONITORING, v.14, no.8, pp.2121 - 2126 JOURNAL OF ENVIRONMENTAL MONITORING 14 8 2121 2126 scie scopus 000306852100012 2-s2.0-84864458667 Chemistry, Analytical Environmental Sciences Chemistry Environmental Sciences & Ecology Article IN-VIVO ANALYSIS ENVIRONMENTAL CHEMICALS HORMONE SYNTHESIS TYROSINE MECHANISM VITRO RATS IDENTIFICATION THIOCYANATE IODINATION