Kim, Da Yeon Kwon, Doo Yeon Lee, Bit Na Seo, Hyo Won Kwon, Jin Seon Lee, Bong Han, Dong Keun Kim, Jae Ho Min, Byoung Hyun Park, Kinam Kim, Moon Suk 2024-01-20T14:30:22Z 2024-01-20T14:30:22Z 2021-09-04 2012-08 1744-683X https://pubs.kist.re.kr/handle/201004/129034 Here, we describe the preparation of microcapsule formulations using in situ-forming hydrogels to achieve desired therapeutic levels over a specific period. Bovine serum albumin (BSA)-fluorescein isothiocyanate (FITC)-loaded microcapsules were prepared using a mono-axial nozzle ultrasonic atomizer with an encapsulation efficiency of approximately 65% and a particle size of approximately 60 mu m. Injectable formulations were prepared by mixing BSA-FITC-loaded microcapsules (Cap) and chitosan (CH), Pluronic (PL), or methoxy poly(ethylene glycol)-b-poly(epsilon-caprolactone) (MPEG-b-PCL) solution (MP). All formulations were prepared as solutions and became gelatinous drug depot implants after injection into the subcutaneous tissue of Sprague-Dawley (SD) rats. While monitoring in vivo BSA release, we found that the initial burst release of BSA was retarded by in situ-forming hydrogels. The T-max and C-max values for each formulation were significantly higher and lower, respectively, than those of the BSA-FITC-solution alone. The absolute bioavailability of BSA-FITC from each formulation depended on the viscosities of the in situ-forming hydrogels. The viscosities of the in situ-forming hydrogels were considered to be an important factor influencing the initial burst and duration of BSA release over a period of several weeks. One conclusion that might be drawn from this work is that the initial burst and sustained entire release profile depend on the hydrogel properties. In conclusion, we believe the results of the present study provide potential new insights into sustained pharmacological performance and represent a useful experimental platform using in situ-forming hydrogels for future protein delivery research. English ROYAL SOC CHEMISTRY OSTEOGENIC DIFFERENTIATION VIVO EFFICACY STEM-CELLS DELIVERY GEL FABRICATION PACLITAXEL ALGINATE RELEASE PLGA Injectable in situ-forming hydrogels for a suppression of drug burst from drug-loaded microcapsules Article 10.1039/c2sm25566a 1 SOFT MATTER, v.8, no.29, pp.7638 - 7648 SOFT MATTER 8 29 7638 7648 scie scopus 000305981600016 2-s2.0-84869595528 Chemistry, Physical Materials Science, Multidisciplinary Physics, Multidisciplinary Polymer Science Chemistry Materials Science Physics Polymer Science Article OSTEOGENIC DIFFERENTIATION VIVO EFFICACY STEM-CELLS DELIVERY GEL FABRICATION PACLITAXEL ALGINATE RELEASE PLGA