Kim, Sang Min Kang, Suk-Woo Kwon, O-Nam Chung, Donghwa Pan, Cheol-Ho 2024-01-20T14:30:25Z 2024-01-20T14:30:25Z 2021-09-04 2012-08 1738-2203 https://pubs.kist.re.kr/handle/201004/129037 Fucoxanthin, a main marine carotenoid, in five species of fucoxanthin-containing microalgae, was quantified by high-performance liquid chromatography. Among the studied species, Isochrysis aff. galbana contained the highest amount of fucoxanthin (18.23 mg/g dried sample). This microalga showed good fucoxanthin extraction efficiency under the tested solvents (methanol, ethanol, acetone, and ethyl acetate), with the exception of n-hexane. In addition, most fucoxanthin (similar to 95%) could be extracted by a single extraction in ethanol within 5 min, and only 15% degradation of fucoxanthin was detected during ethanol extraction for 24 h. The two-phase solvent system of n-hexane-ethanol-water with a volume ratio of 10:9:1 was determined to be the best system for the separation of fucoxanthin and lipids from extracts of I. aff galbana. Under these conditions, fucoxanthin was fractionated in the hydroalcohol phase apart from the hexane phase containing lipids. These results imply that I. aff. galbana can be a commercial source for the spontaneous production of valuable fucoxanthins and lipids. English KOREAN SOC APPLIED BIOLOGICAL CHEMISTRY LIPID EXTRACTION MARINE ALGAE COMPLEXES PIGMENTS DIATOMS GROWTH Fucoxanthin as a Major Carotenoid in Isochrysis aff. galbana: Characterization of Extraction for Commercial Application Article 10.1007/s13765-012-2108-3 1 JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY, v.55, no.4, pp.477 - 483 JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY 55 4 477 483 scie scopus kci ART001687516 000309028200005 2-s2.0-84866057611 Food Science & Technology Food Science & Technology Article LIPID EXTRACTION MARINE ALGAE COMPLEXES PIGMENTS DIATOMS GROWTH carotenoid extraction fucoxanthin Isochrysis aff. galbana lipid microalgae two-phase solvent system