Baker, Bradley J. Jin, Lei Han, Zhou Cohen, Lawrence B. Popovic, Marko Platisa, Jelena Pieribone, Vincent 2024-01-20T14:30:59Z 2024-01-20T14:30:59Z 2021-09-05 2012-07-15 0165-0270 https://pubs.kist.re.kr/handle/201004/129063 A substantial increase in the speed of the optical response of genetically encoded fluorescent protein voltage sensors (FP voltage sensors) was achieved by using the voltage-sensing phosphatase genes of Nematostella vectensis and Danio redo. A potential N. vectensis voltage-sensing phosphatase was identified in silico. The voltage-sensing domain (S1-S4) of the N. vectensis homolog was used to create an FP voltage sensor called Nema. By replacing the phosphatase with a cerulean/citrine FRET pair, a new FP voltage sensor was synthesized with fast off kinetics (Tau(off) < 5 ms). However, the signal was small (Delta F/F = 0.4%/200 mV). FP voltage sensors using the D. redo voltage-sensing phosphatase homolog, designated Zahra and Zahra 2, exhibited fast on and off kinetics within 2 ms of the time constants observed with the organic voltage-sensitive dye, di4-ANEPPS. Mutagenesis of the S4 region of the Danio FP voltage sensor shifted the voltage dependence to more negative potentials but did not noticeably affect the kinetics of the optical signal. (C) 2012 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV SQUID GIANT-AXON MEMBRANE VOLTAGE MAMMALIAN-CELLS DYNAMICS PROTEIN PROBE POTENTIALS DYES TIME Genetically encoded fluorescent voltage sensors using the voltage-sensing domain of Nematostella and Danio phosphatases exhibit fast kinetics Article 10.1016/j.jneumeth.2012.05.016 1 JOURNAL OF NEUROSCIENCE METHODS, v.208, no.2, pp.190 - 196 JOURNAL OF NEUROSCIENCE METHODS 208 2 190 196 scie scopus 000306882800014 2-s2.0-84862128124 Biochemical Research Methods Neurosciences Biochemistry & Molecular Biology Neurosciences & Neurology Article SQUID GIANT-AXON MEMBRANE VOLTAGE MAMMALIAN-CELLS DYNAMICS PROTEIN PROBE POTENTIALS DYES TIME Genetically encoded voltage-sensor Fluorescent protein Imaging membrane potential Voltage-sensing phosphatase