Kim, Hye-Jung Kang, Un-Beom Lee, Hanna Jung, Ji-Han Lee, Seung-Taek Yu, Myeong-Hee Kim, Hoguen Lee, Cheolju 2024-01-20T14:32:55Z 2024-01-20T14:32:55Z 2021-09-05 2012-06-06 1874-3919 https://pubs.kist.re.kr/handle/201004/129152 Screening patients at high risk of recurrence of cancer would allow for more accurate and personalized treatment. In this study, we tried to identify the prognosis-related protein profile by applying two different quantitative proteomic techniques, difference in-gel electrophoresis and cleavable isotope-coded affinity tag method. Six tumor tissues were obtained from stage IV colorectal cancer (CRC) patients, of which three have survived more than five years (good prognostic group, GPG) and the other three died within 25 months (poor prognostic group, PPG) after palliative surgery and subsequent chemotherapy treatment. From the two independent quantitative analyses, we identified 175 proteins with abundance ratios greater than 2-fold. Gene ontology analysis revealed that proteins related to cellular assembly/organization and movement were generally increased in the PPG. Twenty-two proteins, including caveolin-1, were chosen for confirmatory studies by Western blot and immunohistochemistry. The Western blot data for each individual protein were analyzed with Mann-Whitney tests, and a multi-marker panel was generated by logistic regression analysis. Five proteins, fatty acid binding protein 1, intelectin 1, transitional endoplasmic reticulum ATPase, transgelin and tropomyosin 2, were significantly different between the two prognostic groups within 95% confidence. No single protein could completely distinguish the two groups from each other. However, a combination of the five proteins effectively distinguished PPG from GPG patients (AUC=1). Our study suggests a multi-marker panel composed of proteome signatures that provide accurate predictive information and potentially assist personalized therapy. This article is part of a Special Issue entitled: Proteomics: The clinical link. (C) 2011 Elsevier B.V. All rights reserved. English ELSEVIER TRANSFORMING GROWTH-FACTOR-BETA-1 QUANTITATIVE-ANALYSIS DISEASE RECURRENCE COLON PROGNOSIS METASTASIS CARCINOMA IDENTIFICATION CAVEOLIN-1 BIOMARKER Profiling of differentially expressed proteins in stage IV Colorectal cancers with good and poor outcomes Article 10.1016/j.jprot.2011.12.002 1 JOURNAL OF PROTEOMICS, v.75, no.10, pp.2983 - 2997 JOURNAL OF PROTEOMICS 75 10 2983 2997 scie scopus 000305266000020 2-s2.0-84861344535 Biochemical Research Methods Biochemistry & Molecular Biology Article TRANSFORMING GROWTH-FACTOR-BETA-1 QUANTITATIVE-ANALYSIS DISEASE RECURRENCE COLON PROGNOSIS METASTASIS CARCINOMA IDENTIFICATION CAVEOLIN-1 BIOMARKER Prognostic biomarker Molecular staging Colorectal cancer DIGE cICAT