Lee, Geumho Choi, Tae Won Kim, Chulwon Nam, Dongwoo Lee, Seok-Geun Jang, Hyeung-Jin Lee, Jun-Hee Um, Jae-Young Jung, Sang Hoon Shim, Bum Sang Ahn, Kyoo Seok Ahn, Kwang Seok 2024-01-20T14:33:42Z 2024-01-20T14:33:42Z 2021-09-05 2012-06 0892-3973 https://pubs.kist.re.kr/handle/201004/129191 Reynoutria elliptica has been used in traditional Korean medicine to promote blood circulation, relieve pain, increase dieresis, and alleviate respiratory problems, through as yet undefined mechanisms. We set out to determine whether the anti-inflammatory effects of this plant are linked with its ability to suppress mitogen-activated protein kinases (MAPKs) and nuclear factor-kappa B (NF-kappa B) activation in lipopolysaccharide (LPS)-activated RAW 264.7 cells. We found for the first time that the hexane fraction of Reynoutria elliptica (HRE) significantly inhibited LPS-stimulated NO and PGE2 synthesis. This is due to the diminishing of the mRNA and protein expression of iNOS and COX-2, respectively. HRE also suppressed LPS-stimulated TNF-alpha secretion in a dose-dependent manner, which might be due to the suppression of LPS-induced MAPKs and NF-kappa B activation. Moreover, our HPLC data demonstrated that the major components of the HRE were bioactive compounds such as emodin-6-Glc, emodin, and physcion. Overall, our results indicate that Reynoutria elliptica could be provided as a potential candidate for anti-inflammation treatment. English INFORMA HEALTHCARE NITRIC-OXIDE SYNTHASE VASCULAR ENDOTHELIAL-CELLS TRANSCRIPTION FACTOR INTERFERON-GAMMA GENE-EXPRESSION P38 MAPK BACTERIAL LIPOPOLYSACCHARIDE DOWN-REGULATION PHORBOL ESTER EMODIN Anti-inflammatory activities of Reynoutria elliptica through suppression of mitogen-activated protein kinases and nuclear factor-kappa B activation pathways Article 10.3109/08923973.2011.619195 1 IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY, v.34, no.3, pp.454 - 464 IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY 34 3 454 464 scie scopus 000303830400014 2-s2.0-84860712887 Immunology Pharmacology & Pharmacy Toxicology Immunology Pharmacology & Pharmacy Toxicology Article NITRIC-OXIDE SYNTHASE VASCULAR ENDOTHELIAL-CELLS TRANSCRIPTION FACTOR INTERFERON-GAMMA GENE-EXPRESSION P38 MAPK BACTERIAL LIPOPOLYSACCHARIDE DOWN-REGULATION PHORBOL ESTER EMODIN Reynoutria elliptica NF-kappa B MAPKs nitric oxide cyclooxygenase-2