Lee, Hye-Jin Kim, Byoung Chan Oh, Min-Kyu Kim, Jungbae 2024-01-20T14:34:14Z 2024-01-20T14:34:14Z 2021-09-04 2012-06 1359-7345 https://pubs.kist.re.kr/handle/201004/129219 Stable enzyme precipitate coatings (EPCs) with high enzyme loading were employed to develop a sensitive and reliable detection protocol, termed EPC-linked aptamer assay (EPC-LAA). EPC-LAA achieved the limit of detection to be 0.5 ng per mL of thrombin (13.5 pM), and maintained a reasonably stable detection signal even after storing all the reagents at 40 degrees C for 66 days. English ROYAL SOC CHEMISTRY PROTEIN-DETECTION NEGATIVE COOPERATIVITY A sensitive and reliable detection of thrombin via enzyme-precipitate-coating-linked aptamer assay Article 10.1039/c2cc30710c 1 CHEMICAL COMMUNICATIONS, v.48, no.48, pp.5971 - 5973 CHEMICAL COMMUNICATIONS 48 48 5971 5973 scie scopus 000304263600003 2-s2.0-84861632731 Chemistry, Multidisciplinary Chemistry Article PROTEIN-DETECTION NEGATIVE COOPERATIVITY Aptamer Thrombin enzyme precipitate coating