Jeon, Je-Seung Kim, Sang-Min Lee, Hee Ju Um, Byung Hun Kim, Hak Ki Kim, Chul Young 2024-01-20T14:34:32Z 2024-01-20T14:34:32Z 2021-08-31 2012-06 1082-6076 https://pubs.kist.re.kr/handle/201004/129234 Prenylated isoflavonoids such as 6,8-diprenylorobol, 6,8-diprenylgenistein and 4'-O-methylalpinumisoflavone were purified from n-hexane extract of C. tricuspidata fruits using centrifugal partition chromatography (CPC) with a n-hexane-ethyl acetate-methanol-water (7: 3: 6: 4, v/v, ascending mode) solvent system in a one-step within 200 min. One-step preparative CPC yielded 22.4 mg of 6,8-diprenylorobol and 26.7 mg of 6,8-diprenylgenistein with 95% and 85% purity, respectively, from 350 mg of crude hexane extract. Further purification by crystallization in methanol yielded 4'-O-methylalpinumisoflavone (9.3 mg) at over 95% purity based on the HPLC analysis. The chemical structures of purified compounds were elucidated by H-1, C-13 NMR, and ESI-MS. English TAYLOR & FRANCIS INC XANTHONES FLAVONOIDS CONSTITUENTS ANTIOXIDANT SEPARATION PREPARATIVE ISOLATION AND PURIFICATION OF PRENYLATED ISOFLAVONOIDS FROM CUDRANIA TRICUSPIDATA FRUITS USING CENTRIFUGAL PARTITION CHROMATOGRAPHY Article 10.1080/10826076.2011.621151 1 JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, v.35, no.11, pp.1607 - 1615 JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 35 11 1607 1615 scie scopus 000305566900011 2-s2.0-84862978577 Biochemical Research Methods Chemistry, Analytical Biochemistry & Molecular Biology Chemistry Article XANTHONES FLAVONOIDS CONSTITUENTS ANTIOXIDANT SEPARATION 4 &apos -O-methylalpinumisoflavone 6,8-diprenylgenistein 6,8-diprenylorobol centrifugal partition chromatography Cudrania tricuspidata prenylated isoflavonoids