Kim, Choong Bang, Jae Hoon Kim, Young Eun Lee, Jin Hyung Kang, Ji Yoon 2024-01-20T14:34:49Z 2024-01-20T14:34:49Z 2021-09-05 2012-05-20 0925-4005 https://pubs.kist.re.kr/handle/201004/129247 This paper suggests a microbead-trapping device to distribute hydrogel beads in an array that encapsulate stem cell aggregates. Only facile pipetting of micro-bead suspension into the chip enabled trapping of hydrogel beads in microwell array within a few minutes. External hydrodynamic flow to hold beads was unnecessary because crescent weir at microwell hold the bead firmly. Tight holding of bead allowed strong washing for on-chip fluorescent staining as well as convenient culture media change. This device was applied to on-chip embryoid body (EB) formation and differentiation of mouse embryonic carcinoma (EC) cells. It is able to provide a new tool for studying the effect of chemical cues on the differentiation of stem cells encapsulated in hydrogel beads. The mouse EC cells encapsulated in the beads were cultured in the microfluidic device for 10 days to observe the effect of retinoic acid (RA) on neuronal differentiation as a model case.These experimental results verified the feasibility of long-term culture and immunostaining assay of stem cell differentiation. This device is expected to be useful tool for observing proliferation or interactions of cells in 3D microenvironment. (C) 2012 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE SA EMBRYOID BODIES CULTURE EFFICIENCY SIZE Stable hydrodynamic trapping of hydrogel beads for on-chip differentiation analysis of encapsulated stem cells Article 10.1016/j.snb.2012.02.008 1 SENSORS AND ACTUATORS B-CHEMICAL, v.166, pp.859 - 869 SENSORS AND ACTUATORS B-CHEMICAL 166 859 869 scie scopus 000305356900121 2-s2.0-84862783912 Chemistry, Analytical Electrochemistry Instruments & Instrumentation Chemistry Electrochemistry Instruments & Instrumentation Article EMBRYOID BODIES CULTURE EFFICIENCY SIZE 3D cell culture Bead trap Cell encapsulation Embryonic body Cell differentiation