Kwak, Bong Seop Kim, Hyun Ok Kim, Jae Hun Lee, Seok Jung, Hyo-Il 2024-01-20T14:34:53Z 2024-01-20T14:34:53Z 2021-09-05 2012-05-15 0956-5663 https://pubs.kist.re.kr/handle/201004/129251 The quantitative analysis of sialic acid (SA) at an erythrocyte membrane is becoming an important clinical parameter in diagnosing cancer and diabetes. In spite of such clinical importance, there are only a few, very expensive, time consuming and complicated quantifying methods established. To solve this problem, we demonstrate a novel and direct measurement technique for SA exposed to the cell membrane using a photothermal biosensing system in which the hemoglobin molecules in the erythrocyte absorb a specific wavelength of photons (532 nm) and convert it to a temperature change. For measuring the quantity of SA, we first modified the sensor surface of a micro-scaled thermometer using phenylboronic acid (PBA) containing a self-assembled monolayer (SAM) to capture the SA-expressing erythrocytes. Second, the sensor surface was thoroughly washed, and when more SA was expressed, tighter association of erythrocytes to the biosensor was expected. Thirdly, blood sample changes in temperature, heated by the 532 nm wavelength laser, were measured by the bottom layer's micron sized platinum thermometer. The temperature changes from the erythrocytes captured on the sensor surface could be estimated by the amount of SA expressed on the erythrocyte membrane. This novel SA analysis system can solve the problems raised by conventional methods such as multiple enzyme reactions and a time consuming process. We expect that this system will help provide a new tool in the quantitative analysis of SA expression level for the diagnosis of diabetes and cancers. (c) 2012 Elsevier B.V. All rights reserved. English ELSEVIER ADVANCED TECHNOLOGY B-16 MELANOMA VARIANTS BUBBLES CELLS Quantitative analysis of sialic acid on erythrocyte membranes using a photothermal biosensor Article 10.1016/j.bios.2012.03.008 1 BIOSENSORS & BIOELECTRONICS, v.35, no.1, pp.484 - 488 BIOSENSORS & BIOELECTRONICS 35 1 484 488 scie scopus 000305036000078 2-s2.0-84860542076 Biophysics Biotechnology & Applied Microbiology Chemistry, Analytical Electrochemistry Nanoscience & Nanotechnology Biophysics Biotechnology & Applied Microbiology Chemistry Electrochemistry Science & Technology - Other Topics Article B-16 MELANOMA VARIANTS BUBBLES CELLS Photothermal Biosensor Erythrocyte Sialic acid (SA) Platinum resistance temperature detector (Pt-RTD) Diabetes