Kim, Unyong Jin, Ming Ji Lee, Jaeick Han, Sang Beom In, Moon Kyo Yoo, Hye Hyun 2024-01-20T15:00:54Z 2024-01-20T15:00:54Z 2021-09-05 2012-05 0731-7085 https://pubs.kist.re.kr/handle/201004/129281 (6aR,10aR)-9-(Hydroxymethyl)-6,6-dimethyl-3-(2-methyloctan-2-yl)-6a,7,10,10a-tetrahydrobenzo[c]chromen-1-ol (HU-210) is a synthetic cannabinoid, with a classical cannabinoid structure similar to Delta(9)-tetrahydrocannabinol (Delta(9)-THC). In this study, the in vitro metabolism of HU-210 was investigated in human liver microsomes to characterize associated phase I metabolites. HU-210 was incubated with human liver microsomes, and the reaction mixture was analyzed using LC-MS/MS. HU-210 was metabolized in human liver microsomes, yielding about 24 metabolites. These metabolites were structurally characterized on the basis of accurate mass analyses and MS/MS fragmentation patterns. The major metabolic route for HU-210 was oxygenation. Metabolites M1-M7 were identified as mono-oxygenated metabolites; MB-M15, mono-hydroxylated metabolites; M16-M20, di-oxygenated metabolites; and M21-M24, di-hydroxylated metabolites. These results provide evidence for in vivo HU-210 metabolism, and they may be applied to the analysis of HU-210 and its relevant metabolites in biological samples. (C) 2012 Elsevier B.V. All rights reserved. English ELSEVIER JWH-018 SYSTEM Tentative identification of phase I metabolites of HU-210, a classical synthetic cannabinoid, by LC-MS/MS Article 10.1016/j.jpba.2012.02.007 1 JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, v.64-65, pp.26 - 34 JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 64-65 26 34 scie scopus 000303074800004 2-s2.0-84862784914 Chemistry, Analytical Pharmacology & Pharmacy Chemistry Pharmacology & Pharmacy Article JWH-018 SYSTEM HU-210 Synthetic cannabinoid Metabolism LC-MS/MS