Jang, Ji-Young Kim, Min-Kyoung Jeon, Yoon-Kyung Joung, Yoon-Ki Park, Ki-Dong Kim, Chul-Woo 2024-01-20T15:02:01Z 2024-01-20T15:02:01Z 2021-09-05 2012-04-30 1226-3613 https://pubs.kist.re.kr/handle/201004/129323 Cancer stem cells (CSCs) are resistant to chemo- and radio-therapy, and can survive to regenerate new tumors. This is an important reason why various anti-cancer therapies often fail to completely control tumors, although they kill and eliminate the bulk of cancer cells. In this study, we determined whether or not adenine nucleotide translocator-2 (ANT2) suppression could also be effective in inducing cell death of breast cancer stem-like cells. A sub-population (SP; CD44(+)/CD24-) of breast cancer cells has been reported to have stem/progenitor cell properties. We utilized the adeno-ANT2 shRNA virus to inhibit ANT2 expression and then observed the treatment effect in a SP of breast cancer cell line. In this study, MCF7, MDA-MB-231 cells, and breast epithelial cells (MCF10A) mesenchymally-transdifferentiated through E-cadherin knockdown were used. ANT2 expression was high in both stem-like cells and non-stem-like cells of MCF7 and MDA-MB-231 cells, and was induced and up-regulated by mesenchymal transdifferentiation in MCF10A cells (MCF10A(EMT)). Knockdown of ANT2 by adeno-shRNA virus efficiently induced apoptotic cell death in the stem-like cells of MCF7 and MDA-MB-231 cells, and MCF10A(EMT). Stemlike cells of MCF7 and MDA-MB-231, and MCF10A(EMT) cells exhibited increased drug (doxorubicin) resistance, and expressed a multi-drug resistant related molecule, ABCG2, at a high level. Adeno-ANT2 shRNA virus markedly sensitized the stem-like cells of MCF7 and MDA-MB-231, and the MCF10A(EMT) cells to doxorubicin, which was accompanied by down-regulation of ABCG2. Our results suggest that ANT2 suppression by adeno-shRNA virus is an effective strategy to induce cell death and increase the chemosensitivity of stem-like cells in breast cancer. English 생화학분자생물학회 TUMOR-GROWTH GENE-EXPRESSION IN-VITRO ANT2 CHEMORESISTANCE INVASIVENESS ISOFORMS FAMILY Adenovirus adenine nucleotide translocator-2 shRNA effectively induces apoptosis and enhances chemosensitivity by the down-regulation of ABCG2 in breast cancer stem-like cells Article 10.3858/emm.2012.44.4.019 1 Experimental & Molecular Medicine, v.44, no.4, pp.251 - 259 Experimental & Molecular Medicine 44 4 251 259 scie scopus kci ART001654212 000303616800001 2-s2.0-84860529329 Biochemistry & Molecular Biology Medicine, Research & Experimental Biochemistry & Molecular Biology Research & Experimental Medicine Article TUMOR-GROWTH GENE-EXPRESSION IN-VITRO ANT2 CHEMORESISTANCE INVASIVENESS ISOFORMS FAMILY ABCG2 protein, human adenine nucleotide translocator 2 drug therapy, combination gene therapy neoplastic stem cells RNA, small interfering