Lee, Eun-Jung Kim, Chulwon Kim, Jin-Young Kim, Sung-Moo Nam, Dongwoo Jang, Hyeung-Jin Kim, Sung-Hoon Shim, Bum Sang Ahn, Kyoo Seok Choi, Seung-Hoon Jung, Sang Hoon Ahn, Kwang Seok 2024-01-20T15:03:13Z 2024-01-20T15:03:13Z 2021-09-05 2012-04 0892-3973 https://pubs.kist.re.kr/handle/201004/129373 Patrinia scabiosaefolia (PS) has been used for curing various types of inflammatory-related disorders. However, the precise mechanism of the anti-inflammatory activity of PS remains unclear. Here, we investigated the anti-inflammatory effects of several fractions isolated from the PS in RAW 264.7 macrophages. The results indicated that the ethyl acetate fraction of PS (EAPS) concentration highly suppressed lipopolysaccharide (LPS)-induced nitric oxide (NO) and IL-6 productions without a cytotoxic effect on RAW 264.7 cells. EAPS inhibited the expressions of LPS-induced iNOS and COX-2 protein and their mRNA in a dose-dependent manner. Particularly, EAPS suppressed the level of nuclear factor-kappa B (NF-kappa B) activity, which was linked with the suppression of LPS-induced phosphorylation of p65 at serine 276 and p65 translocation into nuclei, but not MAPK signaling. In addition, treatment with EAPS inhibited the production of TNF-alpha in LPS-injected mice and suppressed the production of IL-6 and TNF-alpha in LPS-stimulated splenocytes from BALB/c mice. Therefore, we demonstrate here that Patrinia scabiosaefolia potentially inhibits the biomarkers related to inflammation through the blocking of NF-kappa B p65 activation, and it may be a potential therapeutic candidate for the treatment of inflammatory diseases. English TAYLOR & FRANCIS LTD NITRIC-OXIDE SYNTHASE TUMOR-NECROSIS-FACTOR REGULATES ENDOTOXIN RELEASE NUCLEAR-FACTOR INTERFERON-GAMMA GENE-EXPRESSION URSOLIC ACID TNF-ALPHA BACTERIAL LIPOPOLYSACCHARIDE TRANSCRIPTION FACTOR Inhibition of LPS-induced inflammatory biomarkers by ethyl acetate fraction of Patrinia scabiosaefolia through suppression of NF-kappa B activation in RAW 264.7 cells Article 10.3109/08923973.2011.602412 1 IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY, v.34, no.2, pp.282 - 291 IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY 34 2 282 291 scie scopus 000300444800016 2-s2.0-84863131027 Immunology Pharmacology & Pharmacy Toxicology Immunology Pharmacology & Pharmacy Toxicology Article NITRIC-OXIDE SYNTHASE TUMOR-NECROSIS-FACTOR REGULATES ENDOTOXIN RELEASE NUCLEAR-FACTOR INTERFERON-GAMMA GENE-EXPRESSION URSOLIC ACID TNF-ALPHA BACTERIAL LIPOPOLYSACCHARIDE TRANSCRIPTION FACTOR Patrinia scabiosaefolia inflammation NF-kappa B nitric oxide cyclooxygenase-2