Choi, Soon Jung Lee, Min Young Jo, Hyoung Lim, Soon Sung Jung, Sang Hoon 2024-01-20T15:03:29Z 2024-01-20T15:03:29Z 2021-09-05 2012-04 0918-6158 https://pubs.kist.re.kr/handle/201004/129384 In the present study, extracts from Rhus verniciflua were demonstrated to significantly attenuate the negative effects of hydrogen peroxide (H2O2) on transformed retinal ganglion cell line (RGC-5 cells), indicating that they may be protective against oxidative stress-induced retinal degeneration. The inclusion of R. verniciflua in the culture was found to both reduce the levels of reactive oxygen species (ROS) present and lessen the up-regulation of apoptotic proteins such as cleaved poly(ADP-ribose) polymerase, cleaved caspase-3, and cleaved caspase-9. Active compounds were also successfully isolated from R. verniciflua using high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of n-hexane ethyl acetate-methanol-water (3.5:5:3.5:5, v/v). Using this method, we successfully separated 252.1 mg of fustin at a purity of over 93.09%, 51.2 mg of fisetin at a purity of over 95.45%, 39.7 mg of sulfuretin at a purity of over 95.17%, and 10.7 mg of butein at a purity of over 95.01% from 1.5 g of R. vernicifina extract. The chemical structures of these compounds were elucidated by chemical and spectral analyses. There isolated compounds also significantly attenuated the negative effects of H2O2 on RGC-5 cells. Results therefore suggest that, due to its anti-oxidative and anti-apoptotic effects, R. verniciflua could be used as a lead substance for the treatment of retinal diseases such as glaucoma. English PHARMACEUTICAL SOC JAPAN GANGLION-CELL LINE OXIDATIVE STRESS IN-VIVO INDUCED APOPTOSIS EPIGALLOCATECHIN GALLATE LIPID-PEROXIDATION GLAUCOMA STOKES DEATH VITRO Preparative Isolation and Purification of Neuroprotective Compounds from Rhus verniciflua by High Speed Counter-Current Chromatography Article 10.1248/bpb.35.559 1 BIOLOGICAL & PHARMACEUTICAL BULLETIN, v.35, no.4, pp.559 - 567 BIOLOGICAL & PHARMACEUTICAL BULLETIN 35 4 559 567 scie scopus 000302200900017 2-s2.0-84859452692 Pharmacology & Pharmacy Pharmacology & Pharmacy Article GANGLION-CELL LINE OXIDATIVE STRESS IN-VIVO INDUCED APOPTOSIS EPIGALLOCATECHIN GALLATE LIPID-PEROXIDATION GLAUCOMA STOKES DEATH VITRO Rhus verniciflua neuroprotection high-speed counter-current chromatography retinal ganglion cell-5