Lee, Soojin Kim, Borim Park, Kyungmoon Um, Youngsoon Lee, Jinwon 2024-01-20T15:03:45Z 2024-01-20T15:03:45Z 2021-09-05 2012-04 0273-2289 https://pubs.kist.re.kr/handle/201004/129395 meso-2,3-Butanediol (meso-2,3-BDO) is essential for the synthesis of various economically valuable biosynthetic products; however, the production of meso-2,3-BDO from expensive carbon sources is an obstacle for industrial applications. In this study, genes involved in the synthesis of 2,3-BDO in Klebsiella pneumoniae were identified and used to genetically modify Escherichia coli for meso-2,3-BDO production. Two 2,3-BDO biosynthesis genes-budA, encoding acetolactate, and meso-budC, encoding meso-SADH-from K. pneumoniae were cloned into the pUC18 plasmid and introduced into E. coli. In 2 l batch culture, the SGSB03 E. coli strain yielded meso-2,3-BDO at 0.31 g/g(glucose) (with a maximum of 15.7 g/l(culture) after 48 h) and 0.21 g/g(crude glycerol) (with a maximum of 6.9 g/l(culture) after 48 h). Batch cultures were grown under optimized conditions (aerobic, 6% carbon source, 37 A degrees C, and initial pH 7). To find the optimal culture conditions for meso-2,3-BDO production, we evaluated the enzyme activity of meso-SADH and the whole cell conversion yield (meso-2,3-BDO/acetoin) of the E. coli SGSB02, which contains pSB02. meso-SADH showed high enzyme activity at 30-37 A degrees C and pH 7 (30.5-41.5 U/mg of protein), and the conversion yield of SGSB02 E. coli was highest at 37-42 A degrees C and a pH of 7 (0.25-0.28 g (meso-2,3-BDO)/g(acetoin)). English Humana Press, Inc. Synthesis of Pure meso-2,3-Butanediol from Crude Glycerol Using an Engineered Metabolic Pathway in Escherichia coli Article 10.1007/s12010-012-9593-z 1 Applied Biochemistry and Biotechnology, v.166, no.7, pp.1801 - 1813 Applied Biochemistry and Biotechnology 166 7 1801 1813 N scie scopus 000302770700016 2-s2.0-84862802719 Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Article 2 3-BUTANEDIOL meso-2,3-Butanediol Crude glycerol Klebsiella pneumonia Escherichia coli Gene manipulation Metabolic pathway engineering