Koo, Heebeom Moon, Hyungwon Han, Hyounkoo Na, Jin Hee Huh, Myung Sook Park, Jae Hyung Wood, Se Joon Park, Kyu Hyung Kwon, Ick Chan Kim, Kwangmeyung Kim, Hyuncheol 2024-01-20T15:04:02Z 2024-01-20T15:04:02Z 2021-09-04 2012-04 0142-9612 https://pubs.kist.re.kr/handle/201004/129407 The purpose of this study is to determine the correlation between the distribution of nanoparticles in the vitreous and retina and their surface properties after intravitreal injection. For this purpose, we synthesized seven kinds of nanoparticles through self-assembly of amphiphilic polymer conjugates in aqueous condition. They showed similar size but different surface properties. They were labeled with fluorescent dyes for efficient tracking. After intravitreal injection of these nanoparticles into a rodent eye, their time-dependent distribution in the vitreous and retina was determined in stacking tissue images by confocal microscopy. The results demonstrated that the surface property of nanoparticles is a key factor in determining their distribution in the vitreous and retina after intravitreal injection. In addition, immunohistochemistry and TEM images of retina tissues suggested the important mechanism related with Muffler cells for intravitreally administered nanoparticles to overcome the physical barrier of inner limiting membrane and to penetrate into the deeper retinal structures. Therefore, we expect that this study can provide valuable information for biomedical researchers to develop optimized nanoparticles as drug or gene carriers for retinal and optic nerve disorders such as glaucoma, age-related macular degeneration, and diabetic retinopathy. (C) 2012 Elsevier Ltd. All rights reserved. English ELSEVIER SCI LTD GROWTH-FACTOR THERAPY DRUG-DELIVERY MACULAR DEGENERATION TARGETED DELIVERY GENE DELIVERY ORGANIZATION CHITOSAN SYSTEM SIRNA The movement of self-assembled amphiphilic polymeric nanoparticles in the vitreous and retina after intravitreal injection Article 10.1016/j.biomaterials.2012.01.030 1 BIOMATERIALS, v.33, no.12, pp.3485 - 3493 BIOMATERIALS 33 12 3485 3493 scie scopus 000301886300011 2-s2.0-84862782522 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article GROWTH-FACTOR THERAPY DRUG-DELIVERY MACULAR DEGENERATION TARGETED DELIVERY GENE DELIVERY ORGANIZATION CHITOSAN SYSTEM SIRNA Ocular Intravitreal Nanoparticle Drug delivery Vitreous Retina