Oh, Jin Soo Park, In Su Kim, Keung Nyun Yoon, Do Heum Kim, Sang-Heon Ha, Yoon 2024-01-20T15:04:31Z 2024-01-20T15:04:31Z 2021-09-05 2012-03-28 0959-4965 https://pubs.kist.re.kr/handle/201004/129425 We investigated whether transplantation of a three-dimensional cell mass of adipose-derived stem cells (3DCM-ASCs) improved hind limb functional recovery by the stimulation of angiogenesis and neurogenesis in a spinal cord injury. In in-vitro experiments, we confirmed that 3DCM-ASCs differentiated into CD31-positive endothelial cells. To evaluate the therapeutic effect of 3DCM-ASCs in vivo, PBS, human adipose tissue-derived stem cells, and 3DCM-ASCs were transplanted into a spinal cord injury model. The 3DCM-ASCs transplanted into the injured spinal cord differentiated into CD31-positive endothelial cells and remained differentiated. Transplantation of 3DCM-ASCs into the injured spinal cord significantly elevated the density of vascular formations through angiogenic factors released by the 3DCM-ASCs at the lesion site, and enhanced axonal outgrowth at the lesion site. Consistent with these results, the transplantation of 3DCM-ASCs significantly improved functional recovery compared with both ASC transplantation and PBS treatment. These findings suggest that transplantation of 3DCM-ASCs may be an effective stem cell therapy for the treatment of spinal cord injuries and neural ischemia. NeuroReport 23: 277-282 (C) 2012 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins. English LIPPINCOTT WILLIAMS & WILKINS BONE-MARROW ANGIOGENESIS TISSUE VIVO Transplantation of an adipose stem cell cluster in a spinal cord injury Article 10.1097/WNR.0b013e3283505ae2 1 NEUROREPORT, v.23, no.5, pp.277 - 282 NEUROREPORT 23 5 277 282 scie scopus 000301289300003 2-s2.0-84862803079 Neurosciences Neurosciences & Neurology Article BONE-MARROW ANGIOGENESIS TISSUE VIVO angiogenesis spinal cord injury stem cell transplantation three-dimensional cell mass