Lee, Soo Hyun An, Ji Hye Park, Hyun-Mee Jung, Byung Hwa 2024-01-20T15:04:46Z 2024-01-20T15:04:46Z 2021-09-05 2012-03-01 1570-0232 https://pubs.kist.re.kr/handle/201004/129433 Gut microflora are recognized as an active metabolic compartment in whole body systems. Understanding their impact on host physiology is an ongoing process, although many studies demonstrate that they play significant roles in host life. To assess the impact of gut microflora on host physiology in normal or close to normal conditions of the intestine, we prepared pseudo germ-free rats by antibiotic treatment, and we investigated urinary metabolite profiles of pseudo germ-free rats using UPLC-QTOF-MS based on metabolomics. The repeatability and stability of the analysis were evaluated using QC samples and testmixes in both positive and negative ionization modes. When data sets were analyzed with OPLS-DA, 25 metabolites related to the activities of gut microflora were identified. The changes of amino acid metabolism, especially aromatic or sulfur amino acids, and alternations of bioactive nutrients, such as isoflavonoid and riboflavin were observed in the pseudo germ-free rats. Among the sulfur amino acid metabolites, the metabolites reflecting oxidative stress increased in the urine of pseudo germ-free animals, which imply that the activities of intestinal microorganisms can affect the host redox homeostasis. Altered isoflavonoid metabolism due to lack of gut bacteria may impact on steroid hormone metabolism in the body, especially estrogen metabolism. These results indicate that the some essential metabolic pathways are sensitive to the activities of gut microorganism and directly or indirectly affected by the state of intestinal bacteria, thus gut microflora plays an important role in whole body physiology. (c) 2012 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV CHROMATOGRAPHY-MASS SPECTROMETRY FECAL ISOFLAVONE DEGRADATION INFLAMMATORY BOWEL DISEASES GUT TRANSIT-TIME LIQUID-CHROMATOGRAPHY CYSTEINE DIOXYGENASE METABONOMIC ANALYSIS SYSTEMS BIOLOGY SOY ISOFLAVONES UPLC-MS Investigation of endogenous metabolic changes in the urine of pseudo germ-free rats using a metabolomic approach Article 10.1016/j.jchromb.2011.12.030 1 JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v.887, pp.8 - 18 JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 887 8 18 scie scopus 000302049600002 2-s2.0-84862781393 Biochemical Research Methods Chemistry, Analytical Biochemistry & Molecular Biology Chemistry Article CHROMATOGRAPHY-MASS SPECTROMETRY FECAL ISOFLAVONE DEGRADATION INFLAMMATORY BOWEL DISEASES GUT TRANSIT-TIME LIQUID-CHROMATOGRAPHY CYSTEINE DIOXYGENASE METABONOMIC ANALYSIS SYSTEMS BIOLOGY SOY ISOFLAVONES UPLC-MS Gut microbiota Biomarker Global metabolic profiling